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Titolo:
Long-term expansion and maintenance of cord blood haematopoietic stem cells using thrombopoietin, Flt3-ligand, interleukin (IL)-6 and IL-11 in a serum-free and stroma-free culture system
Autore:
Lazzari, L; Lucchi, S; Rebulla, P; Porretti, L; Puglisi, G; Lecchi, L; Sirchia, G;
Indirizzi:
Osped Maggiore, IRCCS, Ctr Trasfus & Immunol Trapianti, Milano Cord Blood Bank, I-20122 Milan, Italy Osped Maggiore Milan Italy I-20122 Cord Blood Bank, I-20122 Milan, Italy
Titolo Testata:
BRITISH JOURNAL OF HAEMATOLOGY
fascicolo: 2, volume: 112, anno: 2001,
pagine: 397 - 404
SICI:
0007-1048(200102)112:2<397:LEAMOC>2.0.ZU;2-0
Fonte:
ISI
Lingua:
ENG
Soggetto:
EX-VIVO EXPANSION; COMBINED IMMUNODEFICIENT MICE; HEMATOPOIETIC PROGENITOR CELLS; FLT3 LIGAND; PLATELET PRODUCTION; SELF-RENEWAL; IN-VITRO; TRANSPLANTATION; AMPLIFICATION; REPOPULATION;
Keywords:
cord blood expansion; thrombopoietin; interleukin 6; interleukin 11; Flt3-ligand;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
38
Recensione:
Indirizzi per estratti:
Indirizzo: Lazzari, L Osped Maggiore, IRCCS, Ctr Trasfus & Immunol Trapianti, Milano Cord Blood Bank, Via Francesco Sforza 35, I-20122 Milan, Italy Osped Maggiore Via Francesco Sforza 35 Milan Italy I-20122 aly
Citazione:
L. Lazzari et al., "Long-term expansion and maintenance of cord blood haematopoietic stem cells using thrombopoietin, Flt3-ligand, interleukin (IL)-6 and IL-11 in a serum-free and stroma-free culture system", BR J HAEM, 112(2), 2001, pp. 397-404

Abstract

Although cord blood (CB) compares favourably with other haematopoietic stem cell (HSCs) sources, its use in large patients is limited by the low number of cells available. Ex vivo expansion of CB HSCs has been used to overcome this limitation. In this study, we investigated the effect of different cytokine cocktails, including interleukin (IL)-6, IL-11, Flt3-ligand (FL) and thrombopoietin (TPO) combined with serum or serum-free medium on the ex vivo expansion of CD34(+) cells from CB. Initial experiments showed that expansion could be slightly improved using serum, but we chose to use serum-free medium in the subsequent investigations to apply good medical practice (GMP) conditions suitable for clinical use. The highest expansion of CD34(+) cells was obtained with a cocktail containing FL + TPO + IL-6 + IL-11. The median (range) fold expansions of CD34(+) cells at 5 and 10 weeks with serum-free medium were 235.6 (131.3-340) and 5205.6 (4736.6-5674.7) respectively. The absence of IL-11 was associated with a similar fold expansion after 5 weeks (median 215.6, range 149.8-281.5), but after 10 weeks expansion was slightly lower (median 1314.7, range 645-1984.4). Our data support the possibility of maintaining long-term expansion of CB HSCs in a simple stroma- and serum-free system.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/04/20 alle ore 09:08:18