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Titolo:
Receptor protection studies to characterize neuronal nicotinic receptors: tubocurarine prevents alkylation of adrenal nicotinic receptors
Autore:
Free, RB; McKay, DB;
Indirizzi:
Ohio State Univ, Coll Pharm, Div Pharmacol, Columbus, OH 43210 USA Ohio State Univ Columbus OH USA 43210 v Pharmacol, Columbus, OH 43210 USA
Titolo Testata:
BRAIN RESEARCH
fascicolo: 1-2, volume: 891, anno: 2001,
pagine: 176 - 184
SICI:
0006-8993(20010209)891:1-2<176:RPSTCN>2.0.ZU;2-Z
Fonte:
ISI
Lingua:
ENG
Soggetto:
BIND ALPHA-BUNGAROTOXIN; BOVINE CHROMAFFIN CELLS; ACETYLCHOLINE-RECEPTORS; CATECHOLAMINE SECRETION; ION CHANNELS; SUBUNITS; SENSITIVITY; SUBTYPES; ANTAGONISTS; VINBLASTINE;
Keywords:
nicotinic receptor; adrenal chromaffin cell; receptor protection assay;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
41
Recensione:
Indirizzi per estratti:
Indirizzo: McKay, DB Ohio State Univ, Coll Pharm, Div Pharmacol, 500 W 12th Ave, Columbus, OH 43210 USA Ohio State Univ 500 W 12th Ave Columbus OH USA 43210 H 43210 USA
Citazione:
R.B. Free e D.B. McKay, "Receptor protection studies to characterize neuronal nicotinic receptors: tubocurarine prevents alkylation of adrenal nicotinic receptors", BRAIN RES, 891(1-2), 2001, pp. 176-184

Abstract

Our laboratory has evidence that multiple nicotinic acetylcholine receptorsubtypes regulate bovine adrenal catecholamine release. In the following studies, receptor protection assays were used to differentiate adrenal nicotinic receptor subpopulations. Under alkylating conditions, bromoacetylcholine (30 muM) reduced nicotinic receptor-stimulated adrenal catecholamine secretion by approximately 80%. When 100 muM tubocurarine was present during alkylation, nicotine-stimulated secretion was reduced by less than 30%. Hexamethonium (500 muM), decamethonium (500 muM), mecamylamine (50 muM), pentolinium (50 muM), adiphenine (50 muM), methyllycaconitine (1 muM) and alpha -bungarotoxin (1 muM) afforded no protection when present during alkylation. When the pharmacology of residual, tubocurarine-protected receptors was investigated, the EC50 value for nicotine's stimulatory effects on secretion significantly increased from 4.0 (2.5-6.5) muM in control cells to 9.1 (7.2-11.4) muM in tubocurarine-protected cells. In addition, the IC50 value fortubocurarine's inhibitory effects on release significantly decreased from 0.7 (0.5-0.9) muM in control cells to 0.3 (0.2-0.4) muM in tubocurarine-protected cells. These studies support the use of protection assays to characterize nicotinic receptor subpopulations. (C) 2001 Elsevier Science B.V. Allrights reserved.

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Documento generato il 13/07/20 alle ore 08:02:03