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Titolo:
Chronic reduction in complex I function alters calcium signaling in SH-SY5Y neuroblastoma cells
Autore:
Sherer, TB; Trimmer, PA; Borland, K; Parks, JK; Bennett, JP; Tuttle, JB;
Indirizzi:
Univ Virginia, Ctr Study Neurodegenerat Dis, Charlottesville, VA 22908 USAUniv Virginia Charlottesville VA USA 22908 Charlottesville, VA 22908 USA Emory Univ, Dept Neurol, Atlanta, GA 30322 USA Emory Univ Atlanta GA USA 30322 Univ, Dept Neurol, Atlanta, GA 30322 USA Univ Virginia, Dept Neurosci, Charlottesville, VA 22908 USA Univ VirginiaCharlottesville VA USA 22908 Charlottesville, VA 22908 USA Univ Virginia, Dept Neurol, Charlottesville, VA 22908 USA Univ Virginia Charlottesville VA USA 22908 Charlottesville, VA 22908 USA
Titolo Testata:
BRAIN RESEARCH
fascicolo: 1-2, volume: 891, anno: 2001,
pagine: 94 - 105
SICI:
0006-8993(20010209)891:1-2<94:CRICIF>2.0.ZU;2-R
Fonte:
ISI
Lingua:
ENG
Soggetto:
SPONTANEOUSLY HYPERTENSIVE RATS; NADH-UBIQUINONE OXIDOREDUCTASE; ELECTRON-TRANSPORT CHAIN; PARKINSONS-DISEASE; SUBSTANTIA-NIGRA; SKELETAL-MUSCLE; SMOOTH-MUSCLE; MITOCHONDRIA; BRAIN; STORE;
Keywords:
rotenone; calcium; reactive oxygen species; mitochondria;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
46
Recensione:
Indirizzi per estratti:
Indirizzo: Tuttle, JB Univ Virginia, Ctr Study Neurodegenerat Dis, Charlottesville, VA 22908 USA Univ Virginia Charlottesville VA USA 22908 ville, VA 22908 USA
Citazione:
T.B. Sherer et al., "Chronic reduction in complex I function alters calcium signaling in SH-SY5Y neuroblastoma cells", BRAIN RES, 891(1-2), 2001, pp. 94-105

Abstract

Sporadic, non-familial Parkinson's disease is characterized by a 15-30% reduction in complex I activity of the electron transport chain. A pharmacological model of reduced complex I activity was created by prolonged treatment of SH-SY5Y cells with low doses (5-20 nM) of rotenone, a selective inhibitor of complex I. Short-term (less than 2 week) exposure to rotenone did not influence calcium signaling, production of reactive oxygen species, or mitochondrial morphology. However, following 2 weeks of rotenone exposure, SH-SY5Y cells showed unusual calcium dynamics, specifically multiple calcium responses to carbachol, a muscarinic agonist. These secondary calcium responses were not seen in control SH-SY5Y cells and were dependent upon calciuminflux. Mitochondrial membrane potential was also reduced in low dose rotenone-treated cells. These results demonstrate that a chronic, partial reduction in complex I activity, such as that seen in Parkinson's disease, can alter cell signaling events and perhaps increase the susceptibility of cellsto calcium overload and subsequent cell death. (C) 2001 Elsevier Science BN. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 19/01/20 alle ore 15:19:46