Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
The RITE assay: Identifying effectors that target the transcription machinery using phage display technology
Autore:
Mazzarelli, JM; Ricciardi, RP;
Indirizzi:
Univ Penn, Philadelphia, PA 19104 USA Univ Penn Philadelphia PA USA 19104Univ Penn, Philadelphia, PA 19104 USA
Titolo Testata:
BIOTECHNIQUES
fascicolo: 2, volume: 30, anno: 2001,
pagine: 380 -
SICI:
0736-6205(200102)30:2<380:TRAIET>2.0.ZU;2-7
Fonte:
ISI
Lingua:
ENG
Soggetto:
BINDING PROTEIN-ALPHA; HUMAN TAF(II)135; RETINOIC ACID; E1A; ACTIVATION; PROMOTER; COMPLEX; RAR;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
17
Recensione:
Indirizzi per estratti:
Indirizzo: Ricciardi, RP Univ Penn, Levy Res Bldg,Room 221,4010 Locust St, Philadelphia, PA 19104 USA Univ Penn Levy Res Bldg,Room 221,4010 Locust St Philadelphia PA USA 19104
Citazione:
J.M. Mazzarelli e R.P. Ricciardi, "The RITE assay: Identifying effectors that target the transcription machinery using phage display technology", BIOTECHNIQU, 30(2), 2001, pp. 380

Abstract

We describe an approach using phage display to identify effecters (activators and repressors) of transcription based an the particular component of the general transcription machinery that they target. We refer to this approach as the reverse identification of transcriptional effecters (RITE) assay. A library of phages containing cDNA-encoded peptides displayed on their surfaces is screened using as the target a specific region of one of the general transcription factors (e.g., the C terminus of hTAF(II)135). The aminoacid sequence encoded by the cDNA of an interacting phage is determined and analyzed in a database homology search to identify known or novel factorsthat may interact with the target protein. Candidate effectors from the homology search are synthesized from recombinant clones and tested for their abilities to bind to the target protein and to functionally modulate transcription in vivo, when co-expressed with the transcriptional target protein. Because the RITE assay is a direct measure of the interactions between general transcription proteins and their effecters, it has an advantage over the well-known yeast two-hybrid system, which is nor amenable to identifyingtranscription factor interactions.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/03/20 alle ore 12:02:23