Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
High-throughput methods for detection of genetic variation
Autore:
Kristensen, VN; Kelefiotis, D; Kristensen, T; Borresen-Dale, AL;
Indirizzi:
Norwegian Radium Hosp, Inst Canc Res, N-0310 Oslo, Norway Norwegian RadiumHosp Oslo Norway N-0310 t Canc Res, N-0310 Oslo, Norway Fdn Res & Technol Hellas, Heraklion, Crete, Greece Fdn Res & Technol Hellas Heraklion Crete Greece Heraklion, Crete, Greece Univ Oslo, Oslo, Norway Univ Oslo Oslo NorwayUniv Oslo, Oslo, Norway
Titolo Testata:
BIOTECHNIQUES
fascicolo: 2, volume: 30, anno: 2001,
pagine: 318 -
SICI:
0736-6205(200102)30:2<318:HMFDOG>2.0.ZU;2-C
Fonte:
ISI
Lingua:
ENG
Soggetto:
DENSITY OLIGONUCLEOTIDE ARRAYS; SINGLE-NUCLEOTIDE POLYMORPHISMS; STRAND CONFORMATION POLYMORPHISM; ENERGY-TRANSFER DETECTION; FLIGHT MASS-SPECTROMETRY; OLIGO BASE EXTENSION; MUTATION DETECTION; PRIMER EXTENSION; GENOMIC DNA; POINT MUTATIONS;
Tipo documento:
Review
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
72
Recensione:
Indirizzi per estratti:
Indirizzo: Kristensen, VN Norwegian Radium Hosp, Inst Canc Res, N-0310 Oslo, Norway Norwegian Radium Hosp Oslo Norway N-0310 310 Oslo, Norway
Citazione:
V.N. Kristensen et al., "High-throughput methods for detection of genetic variation", BIOTECHNIQU, 30(2), 2001, pp. 318

Abstract

Understanding human genetic variation is currently believed to reveal the cause of individual susceptibility to disease and the large variation observed in response to treatment. In this review, we will focus on different approaches to identify and visualize genetic alterations. The various approaches for allele discrimination are formally systematically divided into (i) enzymatic approaches, in which the properties of different enzymes to discriminate between nucleotides are used (restriction enzymes type II, Cleavaseand Resolvase, DNA polymerase, and ligase); (ii) electrophoretic methods, in which the allele discrimination is based on the difference in mobility in polymeric gels or capillaries (single- and double-stranded conformation assays, heteroduplex analysis, and DNA sequencing); (iii) solid-phase determination of allelic variants, including high-density oligonucleotide arrays for hybridization analysis, minisequencing primer extension analysis, and fiberoptic DNA sensor array; (iv) chromatographic methods such as denaturinghigh-performance liquid chromatography (DHPLC); (v) other physical methodsof discrimination of allelic variants such as mass spectrometry (mass and charge) or fluorescence exchange-based techniques; and (vi) in silico methods such as high-throughput analysis of expressed sequence tag data. The most frequently used techniques and instrumental settings applied in differentcombinations are described, and other methods that are less broadly used but have interesting potentials are discussed.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 23/01/20 alle ore 12:19:02