Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Osmotically induced cytosolic free Ca2+ changes in human neutrophils
Autore:
Morris, MR; Doull, IJM; Hallett, MB;
Indirizzi:
Univ Coll N Wales, Coll Med, Dept Surg, Mol Signalling Grp, Cardiff CF4 4XN, S Glam, Wales Univ Coll N Wales Cardiff S Glam Wales CF4 4XN iff CF4 4XN, S Glam, Wales Univ Coll N Wales, Coll Med, Cyst Fibrosis Unit, Cardiff CF4 4XN, S Glam, Wales Univ Coll N Wales Cardiff S Glam Wales CF4 4XN iff CF4 4XN, S Glam, Wales
Titolo Testata:
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
fascicolo: 1, volume: 1538, anno: 2001,
pagine: 20 - 27
SICI:
0167-4889(20010205)1538:1<20:OICFCC>2.0.ZU;2-L
Fonte:
ISI
Lingua:
ENG
Soggetto:
CYSTIC-FIBROSIS; CALCIUM SIGNALS; ION COMPOSITION; RENAL MEDULLA; INHIBITION; EXOCYTOSIS;
Keywords:
neutrophil; cytosolic free Ca2+ concentration; osmolarity; cystic fibrosis; signalling; imaging; cell shape; (lung damage); (inflammation);
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
23
Recensione:
Indirizzi per estratti:
Indirizzo: Hallett, MB Univ Coll N Wales, Coll Med, Dept Surg, Mol Signalling Grp, Cardiff CF4 4XN, S Glam, Wales Univ Coll N Wales Cardiff S Glam Wales CF4 4XN S Glam, Wales
Citazione:
M.R. Morris et al., "Osmotically induced cytosolic free Ca2+ changes in human neutrophils", BBA-MOL CEL, 1538(1), 2001, pp. 20-27

Abstract

Cytosolic free Ca2+ concentration in neutrophils was measured by ratiometric fluorometry of intercellular fura2. Increasing the extracellular osmolarity, by either NaCl (300-600 mM) or sucrose (600-1200 mM), caused a rise incytosolic free Ca2+ (Delta (max)congruent to 600 nM). This was not due to cell lysis as the cytosolic free Ca2+ concentration was reversed by restoration of isotonicity and a second rise in cytosolic free Ca2+ could be provoked by repeating the change in extracellular osmolarity. Furthermore, the rise in cytosolic free Ca2+ concentration occurred in the absence of extracellular Ca2+, demonstrating that release of intracellular fura2 into the external medium did not occur. The osmotically-induced rise in cytosolic free Ca2+ was not inhibited by either the phospholipase C-inhibitor U73122, or the microfilament inhibitor cytochalasin B, suggesting that neither signalling via inositol tris-phosphate or the cytoskeletal system were involved. However, the rise in cytosolic free Ca2+ may have resulted from a reduction in neutrophil water volume in hyperosmotic conditions. As these rises in cytosolic Ca2+ (Delta (max)congruent to 600 nM) were large enough to provoke changes in neutrophil activity, we propose that conditions which removes cell water may similarly elevate cytosolic free Ca2+ to physiologically important levels. (C) 2001 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 20/01/21 alle ore 11:43:11