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Titolo:
14-3-3 Proteins and a 13-lipoxygenase form associations in a phosphorylation-dependent manner
Autore:
Holtman, WL; Roberts, MR; Wang, M;
Indirizzi:
TNO, UL, Ctr Phytotechnol, Dept Appl Plant Sci, NL-2333 AL Leiden, Netherlands TNO Leiden Netherlands NL-2333 AL nt Sci, NL-2333 AL Leiden, Netherlands Univ York, Dept Biol, Plant Lab, York YO10 5DD, N Yorkshire, England Univ York York N Yorkshire England YO10 5DD O10 5DD, N Yorkshire, England
Titolo Testata:
BIOCHEMICAL SOCIETY TRANSACTIONS
, volume: 28, anno: 2000,
parte:, 6
pagine: 834 - 836
SICI:
0300-5127(200012)28:<834:1PAA1F>2.0.ZU;2-X
Fonte:
ISI
Lingua:
ENG
Soggetto:
14-3-3-PROTEINS; BINDING;
Keywords:
lipid metabolism; protein interactions;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
15
Recensione:
Indirizzi per estratti:
Indirizzo: Holtman, WL TNO, UL, Ctr Phytotechnol, Dept Appl Plant Sci, Wassenaarseweg64, NL-2333AL Leiden, Netherlands TNO Wassenaarseweg 64 Leiden Netherlands NL-2333 AL therlands
Citazione:
W.L. Holtman et al., "14-3-3 Proteins and a 13-lipoxygenase form associations in a phosphorylation-dependent manner", BIOCH SOC T, 28, 2000, pp. 834-836

Abstract

Recently, we have demonstrated by two different methods that lipoxgenases (LOXs) and 14-3-3 proteins form interactions in barley embryos [Holtman, Roberts, Oppedijk, Testerink, van Zeijl and Wang (2000) FEES Lett. 474, 48-52]. It was shown by both co-immunoprecipitations and surface-plasmon resonance experiments that 13-LOX, but not 9-LOX, forms interactions with 14-3-3 proteins. In the present report we show that the presence of 13-LOX and 14-3-3 proteins was established in high-molecular-mass complexes. Amounts of 13-LOX and 14-3-3 proteins in high-molecular-mass fractions increased during germination, but were reduced after dephosphorylation of protein extracts or competition with the 14-3-3-binding peptide P-Raf-259, indicating that 13-LOX and 14-3-3 proteins interact in a phosphorylation-dependent manner.

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Documento generato il 01/12/20 alle ore 16:02:09