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Titolo:
Maturation depresses cGMP-mediated decreases in [Ca2+](i) and Ca2+ sensitivity in ovine cranial arteries
Autore:
Nauli, SM; Zhang, LB; Pearce, WJ;
Indirizzi:
Loma Linda Univ, Sch Med, Ctr Perinatal Biol, Dept Physiol, Loma Linda, CA92350 USA Loma Linda Univ Loma Linda CA USA 92350 Physiol, Loma Linda, CA92350 USA Loma Linda Univ, Sch Med, Ctr Perinatal Biol, Dept Pharmacol, Loma Linda, CA 92350 USA Loma Linda Univ Loma Linda CA USA 92350 armacol, Loma Linda, CA 92350 USA
Titolo Testata:
AMERICAN JOURNAL OF PHYSIOLOGY-HEART AND CIRCULATORY PHYSIOLOGY
fascicolo: 3, volume: 280, anno: 2001,
pagine: H1019 - H1028
SICI:
0363-6135(200103)280:3<H1019:MDCDI[>2.0.ZU;2-0
Fonte:
ISI
Lingua:
ENG
Soggetto:
DEPENDENT PROTEIN-KINASE; CEREBRAL-ARTERIES; CALCIUM SENSITIVITY; CAROTID ARTERIES; RECEPTOR; CAMP;
Keywords:
8-(p-chlorophenylthio)-guanosine 3 ',5 '-cyclic monophosphate; cerebral arteries; cerebrovascular circulation; guanylate cyclase; intracellular calcium concentration;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
33
Recensione:
Indirizzi per estratti:
Indirizzo: Pearce, WJ Loma Linda Univ, Sch Med, Ctr Perinatal Biol, Dept Physiol, Loma Linda, CA92350 USA Loma Linda Univ Loma Linda CA USA 92350 oma Linda, CA92350 USA
Citazione:
S.M. Nauli et al., "Maturation depresses cGMP-mediated decreases in [Ca2+](i) and Ca2+ sensitivity in ovine cranial arteries", AM J P-HEAR, 280(3), 2001, pp. H1019-H1028

Abstract

Because cerebrovascular cGMP levels vary significantly during maturation, we examined the hypothesis that the ability of cGMP to relax cerebral arteries also changes during maturation. In concentration-response experiments, potassium-induced tone in basilar arteries was significantly more sensitiveto a nonmetabolizable cell-permeant cGMP analogue 8-(p-chlorophenylthio)-cGMP (8-pCPT-cGMP) in term fetal [-log one-half maximal concentration (EC50)= 4.4 +/- 0.1 M] than in adult (-log EC50 = 4.0 +/- 0.1 M) ovine basilar arteries. Serotonin-induced tone also revealed significantly greater sensitivity to the cGMP analogue in fetal (-log EC50 = 4.9 +/- 0.1 M) than in adult (-log EC50 = 4.7 +/- 0.1 M) basilars. In fura 2-loaded preparations, 8-pCPT-cGMP had no significant effect on cytosolic calcium concentrations in potassium-contracted arteries but at 6 muM significantly reduced calcium onlyin fetal basilars (Delta = 33 +/- 8%). Higher 8-pCPT-cGMP concentrations reduced cytosolic calcium in both fetal and adult basilars. Similarly, in both potassium- and 5-hydroxytryptamine (5-HT)-contracted preparations, low concentrations of 8-pCPT-cGMP reduced myofilament calcium sensitivity only in fetal basilars (Delta = 29 +/- 6 and Delta = 42 +/- 10%, respectively), whereas higher concentrations reduced calcium sensitivity in both fetal and adult arteries. In beta -escin-permeabilized arteries, equivalent reductions in basal and agonist-enhanced myofilament calcium sensitivity were produced by much lower 8-pCPT-cGMP concentrations in fetal (172 and 61 muM, respectively) than in adult (410 and 231 muM, respectively) basilars. The mechanisms mediating cGMP-induced vasorelaxation appear similar in fetal and adult arteries, with the exception that they are much more sensitive to cGMP infetal than adult arteries. These age-related differences in the sensitivity of cytosolic calcium concentration, basal, and agonist-enhanced myofilament calcium sensitivity to cGMP can easily explain why both potassium- and 5-HT-induced tone are more sensitive to cGMP in fetal than adult cerebral arteries.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/03/20 alle ore 09:37:00