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Titolo:
Transient vs. prolonged histone hyperacetylation: effects on colon cancer cell growth, differentiation, and apoptosis
Autore:
Wu, JT; Archer, SY; Hinnebusch, B; Meng, SF; Hodin, RA;
Indirizzi:
Harvard Univ, Sch Med, Beth Israel Deaconess Med Ctr, Dept Surg, Boston, MA 02215 USA Harvard Univ Boston MA USA 02215 Med Ctr, Dept Surg, Boston, MA 02215 USA
Titolo Testata:
AMERICAN JOURNAL OF PHYSIOLOGY-GASTROINTESTINAL AND LIVER PHYSIOLOGY
fascicolo: 3, volume: 280, anno: 2001,
pagine: G482 - G490
SICI:
0193-1857(200103)280:3<G482:TVPHHE>2.0.ZU;2-5
Fonte:
ISI
Lingua:
ENG
Soggetto:
CHAIN FATTY-ACIDS; LARGE-BOWEL CANCER; TRICHOSTATIN-A; DIETARY FIBER; DEACETYLASE INHIBITORS; GENE-EXPRESSION; P21 WAF1/CIP1; HT-29 CELLS; BUTYRATE; INDUCTION;
Keywords:
sodium butyrate; trichostatin A; cyclin-dependent kinase inhibitor; alkaline phosphatase; sodium-potassium-chlorine cotransporter;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
50
Recensione:
Indirizzi per estratti:
Indirizzo: Archer, SY Harvard Univ, Sch Med, Beth Israel Deaconess Med Ctr, Dept Surg, 330 Brookline Ave, Boston, MA 02215 USA Harvard Univ 330 Brookline Ave Boston MA USA 02215 MA 02215 USA
Citazione:
J.T. Wu et al., "Transient vs. prolonged histone hyperacetylation: effects on colon cancer cell growth, differentiation, and apoptosis", AM J P-GAST, 280(3), 2001, pp. G482-G490

Abstract

The role of histone hyperacetylation in regard to growth, differentiation,and apoptosis in colon cancer cells was assessed in an in vitro model system. HT-29 cells were grown in +/-10% fetal bovine serum with either 5 mM sodium butyrate or 0.3 muM trichostatin A [single dose (T) or 3 doses 8 h apart (TR)] for 24 h. Serum-starved HT-29 cells were further treated with epidermal growth factor or insulin-like growth factor I for an additional 24 h. Apoptosis was quantified with propidium iodide and characterized by electron microscopy. Northern blot analyses were performed with cDNA probes specific for intestinal alkaline phosphatase, Na-K-2Cl cotransporter, the cell cycle inhibitor p21, and the actin control. Flow cytometric analysis revealed a time-dependent growth suppression along with early induction of p21 mRNA in the butyrate, T, and TR groups. Histone hyperacetylation, assessed by acid-urea-triton gel electrophoresis, was transient in the T group but persisted for up to 24 h in the butyrate and TR groups. Induction of apoptosis,growth factor unresponsiveness, and differentiation occurred in the butyrate- and TR-treated cells but not those treated with a single dose of trichostatin A. Thus transient hyperacetylation of histones is sufficient to induce p21 expression and produce cellular growth arrest, but prolonged histonehyperacetylation is required for induction of the programs of differentiation, apoptosis, and growth factor unresponsiveness.

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Documento generato il 09/04/20 alle ore 07:40:55