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Titolo:
In vitro assays of vesicular transport
Autore:
Cook, NR; Davidson, HW;
Indirizzi:
Univ Cambridge, Addenbrookes Hosp, Wellcome Trust, Ctr Mol Mechanisms Dis,Cambridge CB2 2XY, England Univ Cambridge Cambridge England CB2 2XY Dis,Cambridge CB2 2XY, England
Titolo Testata:
TRAFFIC
fascicolo: 1, volume: 2, anno: 2001,
pagine: 19 - 25
SICI:
1398-9219(200101)2:1<19:IVAOVT>2.0.ZU;2-7
Fonte:
ISI
Lingua:
ENG
Soggetto:
CELL-FREE SYSTEM; INTERCOMPARTMENTAL PROTEIN-TRANSPORT; RECEPTOR-MEDIATED ENDOCYTOSIS; PERMEABILIZED MDCK CELLS; GTP-BINDING PROTEIN; SEMI-INTACT CELLS; ENDOPLASMIC-RETICULUM; IN-VITRO; GOLGI NETWORK; PLASMA-MEMBRANE;
Keywords:
broken cell; cell free; membrane fusion; vesicle budding; vesicular transport;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
83
Recensione:
Indirizzi per estratti:
Indirizzo: Davidson, HW Univ Cambridge, Addenbrookes Hosp, Wellcome Trust, Ctr Mol Mechanisms Dis,Wellcome Trust MRC Bldg,Hills Rd, Cambridge CB2 2XY, England Univ Cambridge Wellcome Trust MRC Bldg,Hills Rd Cambridge England CB2 2XY
Citazione:
N.R. Cook e H.W. Davidson, "In vitro assays of vesicular transport", TRAFFIC, 2(1), 2001, pp. 19-25

Abstract

Movement of proteins and lipids between the various compartments of eukaryotic cells is fundamental to the maintenance of cellular homeostasis. and an understanding of the molecular mechanisms that govern these processes remains a key goal of cell biological research. This aim has been greatly facilitated by the development of assays that recapitulate specific events in vitro. In the following article we provide an overview of some of the currently used assays that measure the movement of proteins within the exocytic and endocytic pathways, and provide a starting point for those wishing to establish their own systems to study other vesicular transport steps.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 23/01/21 alle ore 03:46:28