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Titolo:
A high-resolution genetic map and a diagnostic RFLP marker for the Mlg resistance locus to powdery mildew in barley
Autore:
Kurth, J; Kolsch, R; Simons, V; Schulze-Lefert, P;
Indirizzi:
John Innes Ctr Plant Sci Res, Sainsbury Lab, Norwich NR4 7UH, Norfolk, England John Innes Ctr Plant Sci Res Norwich Norfolk England NR4 7UH olk, England Rhein Westfal TH Aachen, D-52074 Aachen, Germany Rhein Westfal TH Aachen Aachen Germany D-52074 , D-52074 Aachen, Germany
Titolo Testata:
THEORETICAL AND APPLIED GENETICS
fascicolo: 1, volume: 102, anno: 2001,
pagine: 53 - 60
SICI:
0040-5752(200101)102:1<53:AHGMAA>2.0.ZU;2-J
Fonte:
ISI
Lingua:
ENG
Soggetto:
RACE-SPECIFIC RESISTANCE; F SP HORDEI; PATHOGEN RESISTANCE; RICE; CONSTRUCTION; AVIRULENCES; RESPONSES; PROTEINS; INTERVAL; CLONING;
Keywords:
high-resolution map; powdery mildew fungus; CAPS; resistance gene; diagnostic marker;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
39
Recensione:
Indirizzi per estratti:
Indirizzo: Schulze-Lefert, P John Innes Ctr Plant Sci Res, Sainsbury Lab, Norwich NR47UH, Norfolk, England John Innes Ctr Plant Sci Res Norwich Norfolk England NR4 7UH
Citazione:
J. Kurth et al., "A high-resolution genetic map and a diagnostic RFLP marker for the Mlg resistance locus to powdery mildew in barley", THEOR A GEN, 102(1), 2001, pp. 53-60

Abstract

The semi-dominantly acting Mig resistance locus in barley confers race-specific resistance to the obligate biotrophic fungus Erytsiphe graminis f.sp,hordei. A high-resolution genetic map was constructed at Mig based on a cross between the near-isogenic barley lines Pallas BC5 Mig and Pallas mlg. Atotal of 2000 F-2 progeny were inspected by cleaved amplified polymorphic sequence (CAPS) analysis, defining a 4.47 cM interval encompassing the resistance locus. Pathogen challenge of the segregants with multiple powdery mildew isolates uncovered a novel resistance specificity in Pallas BCS Mig. Probes from within 4.0 cM of Mig were mapped in rice, revealing orthologues on five different rice chromosomes and suggesting multiple breaks of chromosomal collinearity in this region between the two grass species. The most tightly Mig-linked RFLP marker, MWG032, was shown to reliably detect the presence of the resistance allele in a collection of 30 European barley cultivars.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 20/09/20 alle ore 10:40:32