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Titolo:
Caspase inhibitors attenuate oxyhemoglobin-induced apoptosis in endothelial cells
Autore:
Meguro, T; Chen, B; Parent, AD; Zhang, JH;
Indirizzi:
Univ Mississippi, Med Ctr, Dept Neurosurg, Jackson, MS 39216 USA Univ Mississippi Jackson MS USA 39216 pt Neurosurg, Jackson, MS 39216 USA
Titolo Testata:
STROKE
fascicolo: 2, volume: 32, anno: 2001,
pagine: 561 - 566
SICI:
0039-2499(200102)32:2<561:CIAOAI>2.0.ZU;2-P
Fonte:
ISI
Lingua:
ENG
Soggetto:
EXPERIMENTAL SUBARACHNOID HEMORRHAGE; MAJOR CEREBRAL-ARTERIES; DEATH GENE CED-3; NITRIC-OXIDE; INTERLEUKIN-1-BETA-CONVERTING ENZYME; BARRIER DISRUPTION; VASOSPASM; PROTEASES; ACTIVATION; HEMOGLOBIN;
Keywords:
apoptosis; caspase; cerebral ischemia, transient; endothelium; oxyhemoglobins;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
50
Recensione:
Indirizzi per estratti:
Indirizzo: Zhang, JH Univ Mississippi, Med Ctr, Dept Neurosurg, 2500 N State St, Jackson, MS 39216 USA Univ Mississippi 2500 N State St Jackson MS USA 39216 39216 USA
Citazione:
T. Meguro et al., "Caspase inhibitors attenuate oxyhemoglobin-induced apoptosis in endothelial cells", STROKE, 32(2), 2001, pp. 561-566

Abstract

Background and Purpose-Our recent study showed that oxyhemoglobin (OxyHb) induces apoptosis in cultured endothelial cells. Apoptosis requires the action of various classes of proteases, including a family of cysteine proteases known collectively as the caspases, This study was undertaken to investigate the effect of 2 caspase inhibitors, Z-VDVAD-FMK and Z-DEVD-FMK, ill the protection of endothelial cells from OxyHb-induced apoptosis. Methods-Cultured bovine brain microvascular endothelial cells (passages 5 to 9) were exposed to OxyHb (10 mu mol/L) for 24 to 72 hours with and without caspase inhibitors. Cell attachment, DNA ladder, Western blotting of poly(ADP-ribose) polymerase (PARP), and caspase activities were measured to confirm the cytotoxic effect of OxyHb and the protective effect of the caspase inhibitors. Results-(1) OxyHb produced cell detachment in a time-dependent manner. (2)OxyHb increased caspase-2 and -3 activities, produced DNA ladders, and cleaved PARP in endothelial cells, (3) Z-VDVAD-FMK and Z-DEVD-FMK (100 mu mol/L) attenuated OxyHb-induced cell detachment, reduced caspase-2 and -3 activities, abolished OxyHb-induced DNA ladders, and prevented OxyHb-induced cleavage of PARP. Conclusions-OxyHb activates caspase-2 and -3 in cultured brain microvesselendothelial cells. Caspase inhibitors attenuated the cytotoxic effect of OxyHb.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/12/20 alle ore 13:23:28