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Titolo:
Distinct tumour specificity and IL-7 requirements of CD56(-) and CD56(+) subsets of human gamma delta T cells
Autore:
Zheng, B; Lam, C; Im, S; Huang, J; Luk, W; Lau, SY; Yau, KS; Wong, CK; Yao, K; Ng, MH;
Indirizzi:
Univ Hong Kong, Dept Microbiol, Hong Kong, Hong Kong, Peoples R China UnivHong Kong Hong Kong Hong Kong Peoples R China Kong, Peoples R China Queen Mary Hosp, Dept Pathol, Hematol Sect, Hong Kong, Hong Kong, Peoples R China Queen Mary Hosp Hong Kong Hong Kong Peoples R China ong, Peoples R China Nanjing Med Univ, Dept Microbiol, Nanjing, Peoples R China Nanjing Med Univ Nanjing Peoples R China biol, Nanjing, Peoples R China
Titolo Testata:
SCANDINAVIAN JOURNAL OF IMMUNOLOGY
fascicolo: 1, volume: 53, anno: 2001,
pagine: 40 - 48
SICI:
0300-9475(200101)53:1<40:DTSAIR>2.0.ZU;2-J
Fonte:
ISI
Lingua:
ENG
Soggetto:
KILLER INHIBITORY RECEPTORS; HEAT-SHOCK PROTEIN; PERIPHERAL-BLOOD; INFILTRATING LYMPHOCYTES; GLIOBLASTOMA PATIENTS; NONPEPTIDE ANTIGENS; NK CELLS; RECOGNITION; ACTIVATION; LIGANDS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
48
Recensione:
Indirizzi per estratti:
Indirizzo: Ng, MH Univ Hong Kong, Dept Microbiol, Queen Mary Hosp Cpd, Univ Pathol Bldg,Pokfulam Rd, Hong Kong, Hong Kong, Peoples R China Univ Hong Kong Univ Pathol Bldg,Pokfulam Rd Hong Kong Hong Kong Peoples R China
Citazione:
B. Zheng et al., "Distinct tumour specificity and IL-7 requirements of CD56(-) and CD56(+) subsets of human gamma delta T cells", SC J IMMUN, 53(1), 2001, pp. 40-48

Abstract

gamma delta T cells are believed to recognize tissue injury caused by infections, tumours, as well as chemical and physical agents. The present studywas carried out to study the feasibility of the ex vivo expansion of gammadelta T cells from healthy individuals, and to determine their functional capacity against tumours. We selectively expanded the peripheral gamma delta T cells of five donors against a myeloma cell line, XG-7. Under optimal conditions, the resulting bulk cultures comprised about 82% of the gamma delta T cells, more than 90% of which showed the T-cell receptor (TCR)-V gamma9 delta2 rearrangement. These gamma delta T-cell cultures exhibited TCR-gamma delta dependent cytotoxicity against different tumour cell lines including Molt-4, BJAB, Epstein-Barr virus (EBV) transformed lymphoid cell lines (LCL), and the nasopharyngeal carcinoma (NPC) cell lines, CNE2 and 915, in addition to the stimulator XG-7. By competitive cytotoxicity assays, the gamma delta T cells demonstrated recognition of at least three distinct targetspecificities expressed by Molt-4, CNE2 and LCL, respectively, which were related to that expressed by the stimulator XG-7 cells. The recognition of the specificity expressed by XG-7 and Molt-4 was further shown to require the participation of heat shock protein (HSP). The specificity expressed by CNE2 and 915 was preferentially recognized by the CD56(-) subset of gamma delta T cells, which could be sustained in the presence of interleukin (IL)-7. These results suggested that gamma delta T-cell immunity against tumour cell lines may be acquired in response to other types of tissue injury and,hence, implicates a role for their use in the prevention and treatment of tumours.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/10/20 alle ore 09:22:39