Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
High concentrations of dexamethasone suppress the proliferation but not the differentiation or further maturation of human osteoblast precursors in vitro: relevance to glucocorticoid-induced osteoporosis
Autore:
Walsh, S; Jordan, GR; Jefferiss, C; Stewart, K; Beresford, JN;
Indirizzi:
Univ Bath, Sch Pharm & Pharmacol, Bone Res Grp, Bath BA2 7AY, Avon, England Univ Bath Bath Avon England BA2 7AY Res Grp, Bath BA2 7AY, Avon, England Addenbrookes Hosp, Dept Med, Bone Res Grp, Cambridge CB2 2QQ, England Addenbrookes Hosp Cambridge England CB2 2QQ , Cambridge CB2 2QQ, England
Titolo Testata:
RHEUMATOLOGY
fascicolo: 1, volume: 40, anno: 2001,
pagine: 74 - 83
SICI:
1462-0324(200101)40:1<74:HCODST>2.0.ZU;2-1
Fonte:
ISI
Lingua:
ENG
Soggetto:
MARROW STROMAL CELLS; HUMAN BONE-MARROW; GROWTH-FACTOR-BETA; ADULT HUMAN BONE; IN-VITRO; MATRIX PROTEINS; OSTEOGENIC PRECURSORS; ALKALINE-PHOSPHATASE; MONOCLONAL-ANTIBODY; COLLAGEN SYNTHESIS;
Keywords:
glucocorticoid-induced osteoporosis; osteoblasts; bone marrow stromal cells; dexamethasone; colony-forming unit; STRO-1; alkaline phosphatase; flow cytometry; growth factor receptors; integrins;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Citazioni:
57
Recensione:
Indirizzi per estratti:
Indirizzo: Walsh, S Univ Bath, Sch Pharm & Pharmacol, Bone Res Grp, 7 West,Claverton Down, Bath BA2 7AY, Avon, England Univ Bath 7 West,Claverton Down Bath AvonEngland BA2 7AY England
Citazione:
S. Walsh et al., "High concentrations of dexamethasone suppress the proliferation but not the differentiation or further maturation of human osteoblast precursors in vitro: relevance to glucocorticoid-induced osteoporosis", RHEUMATOLOG, 40(1), 2001, pp. 74-83

Abstract

Objective. The use of glucocorticoids (GCs) in the treatment of RA is a frequent cause of bone loss. In vitro, however, this same class of steroids has been shown to promote the recruitment and/or maturation of primitive osteogenic precursors present in the colony forming unit-fibroblastic (CFU-F) fraction of human bone and marrow. In an effort to reconcile these conflicting observations, we investigated the effects of the synthetic GC dexamethasone (Dx) on parameters of growth and osteogenic differentiation in cultures of bone marrow stromal cells derived from a large cohort of adult human donors (n = 30). Methods. Marrow suspensions were cultured in the absence and presence of Ds at concentrations between 10 pM and 1 muM. After 28 days we determined the number and diameter of colonies formed, the total number of cells, the surface expression of receptors for selected growth factors and extracellularmatrix proteins and, based on the expression of thr developmental markers alkaline phosphatase (AP) and the antigen recognized by the STRO-1 monoclonal antibody, the proportion of cells undergoing osteogenic differentiation and their extent of maturation. Results. At a physiologically equivalent concentration, Dr had no effect on the adhesion of CFU-F or on their subsequent proliferation, but did promote their osteogenic differentiation and further maturation. These effects were independent of changes in the expression of the receptors for fibroblast growth factors, insulin-like growth factor 1, nerve growth factor, platelet-derived growth factors and parathyroid hormone:parathyroid hormone-related protein, but were associated with changes in the number of cells expressing the alpha (2) and alpha (4), but not beta (1), integrin subunits. At supraphysiological concentrations, the effects of Dx on the osteogenic recruitment and maturation of CFU-F and their progeny were maintained but at the expense of a decrease in cell number. Conclusions. A decrease in the proliferation of osteogenic precursors, butnot in their differentiation or maturation, is likely to be a key factor in the genesis of GC-induced bone loss.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 21/09/20 alle ore 12:27:35