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Titolo:
Villous trophoblasts cultured on semi-permeable membranes form an effective barrier to the passage of high and low molecular weight particles
Autore:
Hemmings, DG; Lowen, B; Sherburne, R; Sawicki, G; Guilbert, LJ;
Indirizzi:
Univ Alberta, HMRC 6 25, Perinatal Res Ctr, Dept Med Microbiol & Immunol, Edmonton, AB T6G 2H7, Canada Univ Alberta Edmonton AB Canada T6G 2H7 nol, Edmonton, AB T6G 2H7, Canada Univ Alberta, Perinatal Res Ctr, Dept Pharmacol, Edmonton, AB T6G 2H7, Canada Univ Alberta Edmonton AB Canada T6G 2H7 col, Edmonton, AB T6G 2H7, Canada
Titolo Testata:
PLACENTA
fascicolo: 1, volume: 22, anno: 2001,
pagine: 70 - 79
SICI:
0143-4004(200101)22:1<70:VTCOSM>2.0.ZU;2-S
Fonte:
ISI
Lingua:
ENG
Soggetto:
EPIDERMAL GROWTH-FACTOR; HUMAN PLACENTAL TROPHOBLAST; HUMAN TERM PLACENTA; EXTRACELLULAR-MATRIX; EPITHELIAL-CELLS; BEWO CELLS; IN-VITRO; DIFFERENTIATION; INVITRO; CYTOMEGALOVIRUS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
39
Recensione:
Indirizzi per estratti:
Indirizzo: Guilbert, LJ Univ Alberta, HMRC 6 25, Perinatal Res Ctr, Dept Med Microbiol & Immunol, Edmonton, AB T6G 2H7, Canada Univ Alberta Edmonton AB Canada T6G 2H7 , AB T6G 2H7, Canada
Citazione:
D.G. Hemmings et al., "Villous trophoblasts cultured on semi-permeable membranes form an effective barrier to the passage of high and low molecular weight particles", PLACENTA, 22(1), 2001, pp. 70-79

Abstract

An effective in vitro model of the placental villous syncytium cultured onsemi-permeable substrata is essential for studies of infectious pathogen transmission from mother to fetus. Current models using amniotic membranes or thinner artificial membranes show significant leakage, suggesting disruption of tight junctions or the presence of gaps between syncytial units. Such disruption and discontinuity of trophoblast cultures are probably the result of high stromal cell contamination, poor viability and lad; of proliferation in culture. We have successfully cultured confluent layers of tight-junctioned syncytium on semi-permeable insert membranes using highly viable purified cytotrophoblasts and an alternating multiple seeding and differentiation technique. Using criteria including transepithelial diffusion of high and low molecular weight substances, electrical resistance and directional secretion of the matrix metalloproteinase, MMP-9, we demonstrate that these cultures form effective and functional physical barriers that can be maintained for up to 1 month. (C) 2001 Harcourt Publishers Ltd.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 27/09/20 alle ore 07:15:02