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Titolo:
Differential gene expression profiling in human brain tumors
Autore:
Markert, JM; Fuller, CM; Gillespie, GY; Bubien, JK; McLean, LA; Hong, RL; Lee, K; Gullans, SR; Mapstone, TB; Benos, DJ;
Indirizzi:
Univ Alabama, Dept Physiol & Biophys, Birmingham, AL 35294 USA Univ Alabama Birmingham AL USA 35294 & Biophys, Birmingham, AL 35294 USA Univ Alabama, Dept Surg, Birmingham, AL 35294 USA Univ Alabama BirminghamAL USA 35294 Dept Surg, Birmingham, AL 35294 USA Harvard Univ, Brigham & Womens Hosp, Sch Med, Dept Med, Boston, MA 02115 USA Harvard Univ Boston MA USA 02115 Sch Med, Dept Med, Boston, MA 02115 USA Emory Univ, Dept Neurosurg, Atlanta, GA 30322 USA Emory Univ Atlanta GA USA 30322 iv, Dept Neurosurg, Atlanta, GA 30322 USA
Titolo Testata:
PHYSIOLOGICAL GENOMICS
fascicolo: 1, volume: 5, anno: 2001,
pagine: 21 - 33
SICI:
1094-8341(20010207)5:1<21:DGEPIH>2.0.ZU;2-J
Fonte:
ISI
Lingua:
ENG
Soggetto:
MIGRATION INHIBITORY FACTOR; FACTOR MIF; SECONDARY GLIOBLASTOMAS; OLIGONUCLEOTIDE ARRAYS; CDNA MICROARRAYS; NMDA-RECEPTORS; CELL GROWTH; PATTERNS; SUBUNIT; ANGIOGENESIS;
Keywords:
glioblastoma; glutamate transporters; amiloride; NMDA receptors; BNaC; potassium channels; gene microarrays; patch clamp; reverse transcription-polymerase chain reaction; immunohistochemistry;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
60
Recensione:
Indirizzi per estratti:
Indirizzo: Benos, DJ Univ Alabama, Dept Physiol & Biophys, 1918 Univ Blvd,MCLM 704, Birmingham,AL 35294 USA Univ Alabama 1918 Univ Blvd,MCLM 704 Birmingham AL USA 35294 USA
Citazione:
J.M. Markert et al., "Differential gene expression profiling in human brain tumors", PHYSIOL GEN, 5(1), 2001, pp. 21-33

Abstract

Gene expression profiling of three human temporal lobe brain tissue samples (normal) and four primary glioblastoma multiforme (GBM) tumors using oligonucleotide microarrays was done. Moreover, confirmation of altered expression was performed by whole cell patch clamp, immunohistochemical staining, and RT-PCR. Our results identified several ion and solute transport-relatedgenes, such as N-methyl-D-aspartate (NMDA) receptors, alpha -amino-3-hydroxy-5-methyl-4-isoxazole propionate (AMPA)-2 receptors, GABA(A) receptor subunits alpha3, beta1, beta2, and beta3, the glutamate transporter, the glutamate/aspartate transporter II, the potassium channel K(V)2.1, hK(V)beta3, and the sodium/proton exchanger 1 (NHE-1), that are all downregulated in thetumors compared with the normal tissues. In contrast, aquaporin-1, possibly aquaporins-3 and -5, and GLUT-3 message appeared upregulated in the tumors. Our results also confirmed previous work showing that osteopontin, nicotinamide N-methyltransferase, murine double minute 2 (MDM2), and epithelin (granulin) are upregulated in GBMs. We also demonstrate for the first time that the cytokine and p53 binding protein, macrophage migration inhibitory factor (MIF), appears upregulated in GBMs. These results indicate that the modulation of ion and solute transport genes and heretofore unsuspected cytokines (i.e., MIF) may have profound implications for brain tumor cell biology and thus may identify potential useful therapeutic targets in GBMs.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/04/20 alle ore 03:41:23