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Titolo:
Glial tumor cell adhesion is mediated by binding of the FNIII domain of receptor protein tyrosine phosphatase beta (RPTP beta) to tenascin C
Autore:
Adamsky, K; Schilling, J; Garwood, J; Faissner, A; Peles, E;
Indirizzi:
Weizmann Inst Sci, Dept Mol Cell Biol, IL-76100 Rehovot, Israel Weizmann Inst Sci Rehovot Israel IL-76100 Biol, IL-76100 Rehovot, Israel Ctr Neurochim, Unite CNRS UPR 1352, F-67084 Strasbourg, France Ctr Neurochim Strasbourg France F-67084 1352, F-67084 Strasbourg, France Sugen Inc, S San Francisco, CA 94080 USA Sugen Inc S San Francisco CA USA94080 Inc, S San Francisco, CA 94080 USA
Titolo Testata:
ONCOGENE
fascicolo: 5, volume: 20, anno: 2001,
pagine: 609 - 618
SICI:
0950-9232(20010201)20:5<609:GTCAIM>2.0.ZU;2-K
Fonte:
ISI
Lingua:
ENG
Soggetto:
CHONDROITIN SULFATE PROTEOGLYCAN; GROWTH-ASSOCIATED MOLECULE; HIGH-AFFINITY LIGAND; NERVOUS-SYSTEM; HOMOPHILIC BINDING; NEURITE OUTGROWTH; FOCAL ADHESIONS; PTP-MU; ZETA/BETA; EXPRESSION;
Keywords:
tyrosine phosphatase; extracellular matrix; cell adhesion molecule;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
57
Recensione:
Indirizzi per estratti:
Indirizzo: Peles, E Weizmann Inst Sci, Dept Mol Cell Biol, IL-76100 Rehovot, Israel Weizmann Inst Sci Rehovot Israel IL-76100 76100 Rehovot, Israel
Citazione:
K. Adamsky et al., "Glial tumor cell adhesion is mediated by binding of the FNIII domain of receptor protein tyrosine phosphatase beta (RPTP beta) to tenascin C", ONCOGENE, 20(5), 2001, pp. 609-618

Abstract

The extracellular domain of receptor protein tyrosine phosphatase beta (RPTP beta) is composed of several domains which mediate its interactions withdistinct ligands present on the surface of either neurons or glial cells. Here, we demonstrate that the fibronectin type III domain (FNIII) of RPTP beta binds to glial tumor-derived cell lines and primary astrocytes, We usedaffinity purification to isolate several proteins that specifically bind to the FNIII domain of RPTP beta. One of these, a 240 kDa protein that was purified from U118MG glioblastoma cell, was identified as tenascin C based on the amino acid sequence of several tryptic peptides. The interaction of RPTP beta with tenascin C was found to mediate cell adhesion. Adhesion and spreading of SF763T astrocytoma cells expressing RPTP beta on tenascin C,wasspecifically abolished by the addition of a soluble fragment containing the FNIII domain of the receptor, RPTP beta -dependent cell adhesion was mediated by binding to the alternatively spliced FNIII repeats A1,2,4 (TnfnA1,2,4) of tenascin C, Furthermore, COS cells expressing RPTP beta adhere to TnfnA1,2,4, while the parental cells did not. These results demonstrate that the FNIII domain of RPTP beta binds to tenascin C and suggest that RPTP beta present on glial tumor cells is a primary adhesion receptor system to theextracellular matrix.

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Documento generato il 18/01/20 alle ore 21:28:48