Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Amperometric recording of gonadotropin-releasing hormone release activity in the pituitary of the dwarf gourami (teleosat) brain-pituitary slices
Autore:
Ishizaki, M; Oka, Y;
Indirizzi:
Univ Tokyo, Grad Sch Sci, Misaki Marine Biol Stn, Kanagawa 2380225, Japan Univ Tokyo Kanagawa Japan 2380225 rine Biol Stn, Kanagawa 2380225, Japan
Titolo Testata:
NEUROSCIENCE LETTERS
fascicolo: 1-2, volume: 299, anno: 2001,
pagine: 121 - 124
SICI:
0304-3940(20010216)299:1-2<121:AROGHR>2.0.ZU;2-Y
Fonte:
ISI
Lingua:
ENG
Soggetto:
INDIVIDUAL CHROMAFFIN CELLS; ELECTROCHEMICAL DETECTION; COLISA-LALIA; GNRH; NEUROPEPTIDES; EXOCYTOSIS; NEURONS;
Keywords:
gonadotropin-releasing hormone; carbon fiber electrode; electrochemistry; amperometry; pituitary slice; teleost;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
19
Recensione:
Indirizzi per estratti:
Indirizzo: Oka, Y Univ Tokyo, Grad Sch Sci, Misaki Marine Biol Stn, Kanagawa 2380225,Japan Univ Tokyo Kanagawa Japan 2380225 ol Stn, Kanagawa 2380225, Japan
Citazione:
M. Ishizaki e Y. Oka, "Amperometric recording of gonadotropin-releasing hormone release activity in the pituitary of the dwarf gourami (teleosat) brain-pituitary slices", NEUROSCI L, 299(1-2), 2001, pp. 121-124

Abstract

We developed a method for real-time electrochemical recording of gonadotropin-releasing hormone (GnRH) release using carbon fiber electrodes (CFE). The oxidation current of GnRH was measured by amperometry. We recorded the release activity in the pituitary of the teleost brain-pituitary slice. The CFE was located in the part of the pituitary that receives dense innervation from GnRH neurons in the preoptic area. A bulk amperometric current was recorded in response to high [K+](o) stimulation in a dose dependent manner. Decreasing the holding potential to below the oxidation potential of GnRH resulted in the loss of amperometric currents. Thus, it is suggested that the amperometric currents are mainly attributed to the oxidation currents ofGnRH and reflect the GnRH release activity. (C) 2001 Elsevier Science Ireland Ltd. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/04/20 alle ore 02:07:53