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Titolo:
Dialysate dihydroxyphenylglycol as a window for in situ axoplasmic norepinephrine disposition
Autore:
Yamazaki, T; Akiyama, T; Kitagwa, H; Kawada, T; Sunagawa, K;
Indirizzi:
Natl Cardiovasc Ctr, Res Inst, Dept Cardiac Physiol, Osaka 5658565, Japan Natl Cardiovasc Ctr Osaka Japan 5658565 ac Physiol, Osaka 5658565, Japan Natl Cardiovasc Ctr, Res Inst, Dept Cardiovasc Dynam, Osaka 5658565, JapanNatl Cardiovasc Ctr Osaka Japan 5658565 vasc Dynam, Osaka 5658565, Japan
Titolo Testata:
NEUROCHEMISTRY INTERNATIONAL
fascicolo: 3, volume: 38, anno: 2001,
pagine: 287 - 292
SICI:
0197-0186(200103)38:3<287:DDAAWF>2.0.ZU;2-O
Fonte:
ISI
Lingua:
ENG
Soggetto:
MYOCARDIAL INTERSTITIAL NOREPINEPHRINE; LIQUID-CHROMATOGRAPHIC DETERMINATION; HEART; RELEASE; NORADRENALINE; RESPONSES; BRAIN;
Keywords:
cat; catecholamine; heart; microdialysis; noradrenaline; sympathetic nerves;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
28
Recensione:
Indirizzi per estratti:
Indirizzo: Yamazaki, T Natl Cardiovasc Ctr, Res Inst, Dept Cardiac Physiol, Osaka 5658565, Japan Natl Cardiovasc Ctr Osaka Japan 5658565 Osaka 5658565, Japan
Citazione:
T. Yamazaki et al., "Dialysate dihydroxyphenylglycol as a window for in situ axoplasmic norepinephrine disposition", NEUROCHEM I, 38(3), 2001, pp. 287-292

Abstract

To examine basal axoplasmic norepinephrine (NE) kinetics at the in situ cardiac sympathetic nerve ending, we applied a dialysis technique to the heart of anesthetized cats and performed the dialysate sampling with local administration of a pharmacological tool through a dialysis probe. The dialysisprobe was implanted in the left ventricular wall, and dihydroxyphenylglycol (DHPG, an index of axoplasmic NE) levels were measured by liquid chromategram-electro chemical detection. Control dialysate DHPG levels were 161 +/- 19 pg/ml. Pargyline (monoamine oxidase inhibitor, 1 mM) decreased the dialysate DHPG levels to 38 +/- 10 pg/ml, Further alpha -methyl-para-tyrosine,omega -conotoxin GVIA, desipramine (NE synthesis, release and uptake blockers) decreased the dialysate DHPG levels to 64 +/- 19, 106 +/- 15, 110 +/- 22 pg/ml, respectively. In contrast, reserpine (vesicle NE transport inhibitor, 10 muM) increased the dialysate DHPG levels to 690 +/- 42 pg/ml. Thus,NE synthesis, metabolism and recycling (release, uptake and vesicle transport) affected basal intraneuronal NE disposition at the nerve endings. Measurement of DHPG levels through a dialysis probe provides information about basal intraneuronal NE disposition at the cardiac sympathetic nerve endings. Yohimbine (alpha (2)-adrenoreceptor blocker, 10 muM) and U-521 (catechol-O-methyltransferase blocker, 100 muM) did not alter the dialysate DHPG levels. Furthermore, there were no significant differences in the reserpine induced DHPG increment between the presence and absence of desipramine (10 muM) or alpha -methyl-para-tyrosine (100 mg/kg i.p.). These results may be explained by the presence of two axoplasmic pools of NE, filled by NE taken upand synthesized, and by NE overflow from vesicle. The latter pool of NE may be closed to the monoamine oxidase system in the axoplasma. (C) 2001 Elsevier Science Ltd. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 21/09/20 alle ore 16:15:34