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Titolo:
Caenorhabditis elegans auxilin: a J-domain protein essential for clathrin-mediated endocytosis in vivo
Autore:
Greener, T; Grant, B; Zhang, YH; Wu, XF; Greene, LE; Hirsh, D; Eisenberg, E;
Indirizzi:
NHLBI, Cell Biol Lab, NIH, Bethesda, MD 20892 USA NHLBI Bethesda MD USA 20892 I, Cell Biol Lab, NIH, Bethesda, MD 20892 USA Columbia Univ Coll Phys & Surg, Dept Biochem & Mol Biophys, New York, NY 10032 USA Columbia Univ Coll Phys & Surg New York NY USA 10032 w York, NY 10032 USA
Titolo Testata:
NATURE CELL BIOLOGY
fascicolo: 2, volume: 3, anno: 2001,
pagine: 215 - 219
SICI:
1465-7392(200102)3:2<215:CEAAJP>2.0.ZU;2-G
Fonte:
ISI
Lingua:
ENG
Soggetto:
DOUBLE-STRANDED-RNA; UNCOATING ATPASE; GENETIC INTERFERENCE; COATED VESICLES; DISSOCIATION; EXPRESSION; BASKETS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
26
Recensione:
Indirizzi per estratti:
Indirizzo: Eisenberg, E NHLBI, Cell Biol Lab, NIH, Bethesda, MD 20892 USA NHLBI Bethesda MD USA 20892 Lab, NIH, Bethesda, MD 20892 USA
Citazione:
T. Greener et al., "Caenorhabditis elegans auxilin: a J-domain protein essential for clathrin-mediated endocytosis in vivo", NAT CELL BI, 3(2), 2001, pp. 215-219

Abstract

The budding of clathrin-coated vesicles is essential for protein transport. After budding, clathrin must be uncoated before the Vesicles can fuse with other membranous structures. In vitro, the molecular chaperone Hsc70 uncoats clathrin-coated vesicles in an ATP-dependent process that requires a specific J-domain protein such as auxilin. However, there is little evidence that either Hsc70 or auxilin is essential in vivo. Here we show that C. elegans has a single auxilin homologue that is identical to mammalian auxilin in its in vitro activity. When RNA-mediated interference (RNAi) is used to inhibit auxilin expression in C. elegans, oocytes show markedly reduced receptor-mediated endocytosis of yolk protein tagged with green fluorescent protein (GFP). In addition, most of these worms arrest during larval development, exhibit defective distribution of GFP-clathrin in many cell types, andshow a marked change in clathrin dynamics, as determined by fluorescence recovery after photobleaching (FRAP). We conclude that auxilin is required for in vivo clathrin-mediated endocytosis and development in C. elegans.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 20/09/20 alle ore 06:18:33