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Titolo:
The complete DNA sequence of the mitochondrial genome of Physarum polycephalum
Autore:
Takano, H; Abe, T; Sakurai, R; Moriyama, Y; Miyazawa, Y; Nozaki, H; Kawano, S; Sasaki, N; Kuroiwa, T;
Indirizzi:
Kumamoto Univ, Fac Sci, Dept Biol Sci, Kumamoto 8608555, Japan Kumamoto Univ Kumamoto Japan 8608555 t Biol Sci, Kumamoto 8608555, Japan Univ Tokyo, Grad Sch Sci, Dept Biol Sci, Tokyo 113, Japan Univ Tokyo Tokyo Japan 113 Grad Sch Sci, Dept Biol Sci, Tokyo 113, Japan Univ Tokyo, Grad Sch Frontier Sci, Dept Integrated Biosci, Tokyo 113, Japan Univ Tokyo Tokyo Japan 113 Sci, Dept Integrated Biosci, Tokyo 113, Japan
Titolo Testata:
MOLECULAR AND GENERAL GENETICS
fascicolo: 5, volume: 264, anno: 2001,
pagine: 539 - 545
SICI:
0026-8925(200101)264:5<539:TCDSOT>2.0.ZU;2-3
Fonte:
ISI
Lingua:
ENG
Soggetto:
SIMILARITY SEARCH; MESSENGER-RNA; PLASMID; INFORMATION; FUSION; GENES; TOOL;
Keywords:
mitochondria; mtDNA; Physarum polycephalum; RNA editing;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
33
Recensione:
Indirizzi per estratti:
Indirizzo: Takano, H Kumamoto Univ, Fac Sci, Dept Biol Sci, Kumamoto 8608555, Japan Kumamoto Univ Kumamoto Japan 8608555 , Kumamoto 8608555, Japan
Citazione:
H. Takano et al., "The complete DNA sequence of the mitochondrial genome of Physarum polycephalum", MOL G GENET, 264(5), 2001, pp. 539-545

Abstract

The complete sequence of the mitochondrial DNA (mtDNA) of the true slime mold Physarum polycephalum has been determined. The mtDNA is a circular 62,862-bp molecule with an A+T contest of 74.1%. A search with the program BLAST X identified the protein-coding regions. The mitochondrial genome of P. polycephalum was predicted to contain genes coding for 12 known proteins [for three cytochrome ( oxidase subunits, apocytochrome b, two F1Fo-ATPase subunits, five NADH dehydrogenase (nad) subunits, and one ribosomal protein], two rRNA genes, and five tRNA genes. However, the predicted ORFs are not all in the same frame, because mitochondrial RNA in P. polycephalum undergoesRNA editing to produce functional RNAs. The nucleotide sequence of an nad7cDNA showed that 51 nucleotides were inserted at 46 sites in the mRNA. No guide RNA-like sequences were observed in the mtDNA of P. polycephalum. Comparison with reported Physarum mtDNA sequences suggested that sites of RNA editing vary among strains. In the Physarum mtDNA, 20 ORFs of over 300 nucleotides were found and ORFs 14-19 are transcribed.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 27/09/20 alle ore 13:41:01