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Titolo:
Functional characterization and genetic mapping of alk8
Autore:
Payne, TL; Postlethwait, JH; Yelick, PC;
Indirizzi:
Forsyth Inst, Dept Cytokine Biol, Boston, MA 02115 USA Forsyth Inst Boston MA USA 02115 Dept Cytokine Biol, Boston, MA 02115 USA Forsyth Inst, Harvard Forsyth Dept Oral Biol, Boston, MA 02115 USA ForsythInst Boston MA USA 02115 yth Dept Oral Biol, Boston, MA 02115 USA Univ Oregon, Inst Neurosci, Eugene, OR 97403 USA Univ Oregon Eugene OR USA 97403 egon, Inst Neurosci, Eugene, OR 97403 USA
Titolo Testata:
MECHANISMS OF DEVELOPMENT
fascicolo: 2, volume: 100, anno: 2001,
pagine: 275 - 289
SICI:
0925-4773(200102)100:2<275:FCAGMO>2.0.ZU;2-M
Fonte:
ISI
Lingua:
ENG
Soggetto:
GROWTH-FACTOR-BETA; BONE MORPHOGENETIC PROTEINS; EARLY XENOPUS DEVELOPMENT; SERINE/THREONINE KINASE RECEPTOR; DORSOVENTRAL PATTERN-FORMATION; ZEBRAFISH DANIO-RERIO; NO TAIL; WILD-TYPE; SPEMANN ORGANIZER; FAMILY MEMBERS;
Keywords:
zebrafish; transforming growth factor beta family member signaling; activin-like-kinase receptors; bone morphogenetic protein signaling; dorsoventral patterning;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
72
Recensione:
Indirizzi per estratti:
Indirizzo: Yelick, PC Forsyth Inst, Dept Cytokine Biol, 140 Fenway, Boston, MA 02115 USA Forsyth Inst 140 Fenway Boston MA USA 02115 oston, MA 02115 USA
Citazione:
T.L. Payne et al., "Functional characterization and genetic mapping of alk8", MECH DEVEL, 100(2), 2001, pp. 275-289

Abstract

The novel type I TGF beta family member receptor alk8 is expressed both maternally and zygotically. Functional characterization of alk8 was performedusing microinjection studies of constitutively active (CPI), kinase modified/dominant negative (DN), and truncated alk8 mRNAs. CA Alk8 expression produces ventralized embryos while DN Alk8 expression results in dorsalized phenotypes. Truncated alk8 expressing embryos display a subtle dorsalized phenotype closely resembling that of the identified zebrafish dorsalized mutant, lost-a-fin (laf). Single-strand conformation polymorphism (SSCP) analysis was used to map alk8 to zebrafish LG02 in a region demonstrating significant conserved synteny to Hsa2, and which contains the human alk2 gene, ACVRI. Altogether, these functional, gene mapping and phylogenetic analyses suggest that alk8 may be the zebrafish orthologue to human ACVRI (alk2), and therefore extend previous studies of Alk2 conducted in Xenopus. (C) 2001 Elsevier Science Ireland Ltd. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 01/04/20 alle ore 20:12:38