Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Hepatotoxic substance(s) removed by high-flux membranes enhances the positive acute phase response
Autore:
Riegel, W; Ulrich, C; Sauernheimer, S; Deppisch, RM; Kohler, H;
Indirizzi:
Klinikum Darmstadt, D-64276 Darmstadt, Germany Klinikum Darmstadt Darmstadt Germany D-64276 D-64276 Darmstadt, Germany Univ Klin Homburg Saar, Homburg, Germany Univ Klin Homburg Saar Homburg Germany n Homburg Saar, Homburg, Germany
Titolo Testata:
KIDNEY INTERNATIONAL
, volume: 59, anno: 2001, supplemento:, 78
pagine: S308 - S314
SICI:
0085-2538(200102)59:<S308:HSRBHM>2.0.ZU;2-8
Fonte:
ISI
Lingua:
ENG
Soggetto:
CHRONIC-HEMODIALYSIS PATIENTS; CHRONIC-RENAL-FAILURE; ALPHA-1-ACID GLYCOPROTEIN; MORTALITY; SERUM; INFLAMMATION; NUTRITION; REDUCTION; DISEASE; CELLS;
Keywords:
end-stage renal disease; acute phase proteins; uremic toxin; dialysis; hepatocytes; liver; metabolism;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
23
Recensione:
Indirizzi per estratti:
Indirizzo: Riegel, W Klinikum Darmstadt, D-64276 Darmstadt, Germany Klinikum Darmstadt Darmstadt Germany D-64276 armstadt, Germany
Citazione:
W. Riegel et al., "Hepatotoxic substance(s) removed by high-flux membranes enhances the positive acute phase response", KIDNEY INT, 59, 2001, pp. S308-S314

Abstract

Background. Acute phase proteins (APPs) are enhanced in end-stage renal disease patients (ESRD) requiring dialysis treatment. They are involved in a variety of pathologic processes like muscle proteolysis, cachexia, regulation of appetite, and atherosclerosis. They are predictive for mortality. APPs are not only makers but also active substances. They are mainly produced in liver cells and are primarily, but not exclusively, regulated by proinflammatory cytokines. To what extent hepatic APPs are influenced by uremic toxins is still unclear. Therefore, we investigated the effects of different ultrafiltrates (UFs) on the synthesis of alpha (1)-acid glycoprotein (AGP) in HepG2 cells. Methods. A cross-sectional as well as a crossover study with high-/low-flux membranes was conducted to investigate the impact of UFs on bioactivity of liver cell cultures. Metabolic activity (MTT test), cytotoxicity (lactatedehydrogenase release), and the positive APP AGP were measured in HcpG2 cells. Results. Cultured hepatocytes treated with UFs from high-flux membranes exhibited a higher cytotoxicity (18.6 +/- 0.3% high-flux vs. 13.9 +/- 0.2% low-flux, P < 0.001) and a lower metabolic activity (29.3% high-flux vs. 50.3% low-flux, P < 0.001) in comparison with low-flux UFs. In addition, enhanced APP secretion could be observed under costimulatory conditions (high-flux 5.0 +/- 0.7 vs. low-flux 3.1 +/- 0.6 ng/mug protein, P < 0.05). The effects of high- and low-Aux UFs were strongly expressed at the beginning and were still significantly different after 120 minutes of hemodialysis (HD) treatment. The crossover experiments confirmed that UFs collected during high-flux HD had a higher capacity to stimulate AGP synthesis in liver cells. Conclusion. The effects of UFs from dialysis patients demonstrate that hepatotoxic substances can be removed by dialysis. Stimulating the acute phaseresponse UF collected during high-flux HD had a higher impact on liver cells in comparison with low-Rus UF. These substances are putative cofactors involved in cytokine regulation.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/04/20 alle ore 09:42:43