Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Differentiation of stromal-vascular cells isolated from canine adipose tissues in primary culture
Autore:
Wu, PX; Sato, K; Yukawa, S; Hikasa, Y; Kagota, K;
Indirizzi:
Tottori Univ, Fac Agr, Dept Vet Internal Med, Tottori 6808553, Japan Tottori Univ Tottori Japan 6808553 Internal Med, Tottori 6808553, Japan
Titolo Testata:
JOURNAL OF VETERINARY MEDICAL SCIENCE
fascicolo: 1, volume: 63, anno: 2001,
pagine: 17 - 23
SICI:
0916-7250(200101)63:1<17:DOSCIF>2.0.ZU;2-1
Fonte:
ISI
Lingua:
ENG
Soggetto:
ADIPOCYTE PRECURSOR CELLS; NONSTEROIDAL ANTIINFLAMMATORY DRUGS; SERUM-FREE MEDIUM; DIMETHYL-SULFOXIDE; RECEPTOR-GAMMA; DEFINED MEDIUM; HL-60 CELLS; STEM-CELLS; PREADIPOCYTE; INVITRO;
Keywords:
adipocyte differentiation; canine; cell culture; dimethylsulfoxide; indomethacin;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Citazioni:
40
Recensione:
Indirizzi per estratti:
Indirizzo: Sato, K Tottori Univ, Fac Agr, Dept Vet Internal Med, Tottori 6808553, Japan Tottori Univ Tottori Japan 6808553 l Med, Tottori 6808553, Japan
Citazione:
P.X. Wu et al., "Differentiation of stromal-vascular cells isolated from canine adipose tissues in primary culture", J VET MED S, 63(1), 2001, pp. 17-23

Abstract

A culture condition supporting adipocyte differentiation of stromal-vascular (S-V) cells isolated from canine adipose tissues was established. Morphological observation and determination of glycerol-3-phosphate dehydrogenase(GPDH) activity were used as the criteria for adipocyte differentiation. After reaching confluence, the cells were able to undergo terminal adipocytedifferentiation by treatment with 100 muM indomethacin, 10 mug/ml insulin and 0.5 mM 1-methyl-3-isobutylxanthine (MIX) in medium supplemented with 5%fetal calf serum (FCS). In the absence of either indomethacin or insulin, the S-V cells did not undergo adipose conversion and GPDH activity was not increased, indicating that both indomethacin and insulin play essential roles in this culture system. The S-V cells from inguinal adipose tissues exhibited the greatest increase in GPDH activity among the four depots (inguinal > abdominal-subculaneous > perirenal > omental), demonstrating that adipocyte differentiation was also intensely dependent on anatomic sites from which the S-V cells were derived. Interestingly, dimethylsulfoxide (DMSO) wasfound to accelerate adipocyte differentiation in combination with indomethacin and insulin. Under this condition, up to 90% of the cells displayed adipocyte phenotypes and the GPDH activity reached 1288 +/- 441 mU/mg protein. This culture system may be useful for investigating other adipogenic factors as well as anti-adipogenic factors involved in the regulation of canineadipose tissue development.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/12/20 alle ore 01:50:17