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Titolo:
Effects of staurosporine on exocytosis and endocytosis at frog motor nerveterminals
Autore:
Becherer, U; Guatimosim, C; Betz, WJ;
Indirizzi:
Univ Colorado, Sch Med, Dept Physiol & Biophys, Denver, CO 80262 USA Univ Colorado Denver CO USA 80262 Physiol & Biophys, Denver, CO 80262 USA
Titolo Testata:
JOURNAL OF NEUROSCIENCE
fascicolo: 3, volume: 21, anno: 2001,
pagine: 782 - 787
SICI:
0270-6474(20010201)21:3<782:EOSOEA>2.0.ZU;2-E
Fonte:
ISI
Lingua:
ENG
Soggetto:
SYNAPTIC VESICLE ENDOCYTOSIS; PROTEIN-KINASE-C; NEUROMUSCULAR-JUNCTION; CHROMAFFIN CELLS; TRANSMITTER RELEASE; SECRETORY GRANULES; EVOKED RELEASE; CALCIUM; POOL; FLUORESCENCE;
Keywords:
staurosporine; exocytosis; endocytosis; synaptic vesicles; frog neuromuscular junction; FM1-43;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
32
Recensione:
Indirizzi per estratti:
Indirizzo: Betz, WJ Univ Colorado, Sch Med, Dept Physiol & Biophys, Campus Box C-240,Denver, CO 80262 USA Univ Colorado Campus Box C-240 Denver CO USA 80262 , CO 80262 USA
Citazione:
U. Becherer et al., "Effects of staurosporine on exocytosis and endocytosis at frog motor nerveterminals", J NEUROSC, 21(3), 2001, pp. 782-787

Abstract

Observations of the dynamic staining and destaining of FM1-43 in frog motor nerve terminals (Henkel and Betz, 1995) suggested that staurosporine might shorten the interval between exocytosis and endocytosis, inducing a "kissand run" mode of exocytosis and endocytosis. We tested this hypothesis by using FM1-43 imaging (to measure the time course of FM1-43 endocytosis), intracellular recording of evoked synaptic potentials (to measure acetylcholine release), and electron microscopy (to examine synaptic vesicle distribution). Staurosporine reduced FM1-43 uptake during but not after a tetanus, increased the speed of end plate potential (EPP) amplitude rundown, and greatly slowed the recovery from synaptic depression. Ultrastructural observations showed pronounced vesicle depletion near active zones after tetanic stimulation in staurosporine-treated preparations. These results suggest that staurosporine acted primarily to impair mobilization of synaptic vesicles during tetanic stimulation.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 09/04/20 alle ore 19:46:52