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Titolo:
Methamphetamine-induced rapid and reversible changes in dopamine transporter function: An in vitro model
Autore:
Sandoval, V; Riddle, EL; Ugarte, YV; Hanson, GR; Fleckenstein, AE;
Indirizzi:
Univ Utah, Dept Pharmacol & Toxicol, Salt Lake City, UT 84112 USA Univ Utah Salt Lake City UT USA 84112 xicol, Salt Lake City, UT 84112 USA
Titolo Testata:
JOURNAL OF NEUROSCIENCE
fascicolo: 4, volume: 21, anno: 2001,
pagine: 1413 - 1419
SICI:
0270-6474(20010215)21:4<1413:MRARCI>2.0.ZU;2-T
Fonte:
ISI
Lingua:
ENG
Soggetto:
PROTEIN-KINASE-C; TYROSINE-HYDROXYLASE ACTIVITY; RAT-BRAIN; STRIATAL SYNAPTOSOMES; AMPHETAMINE; CLONING; MDMA; 3,4-METHYLENEDIOXYMETHAMPHETAMINE; PHOSPHORYLATION; NEUROTOXICITY;
Keywords:
in vitro; rapid and reversible; serotonin; norepinephrine; transport; phosphorylation;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
39
Recensione:
Indirizzi per estratti:
Indirizzo: Fleckenstein, AE Univ Utah, Dept Pharmacol & Toxicol, 30 S 2000 East,Room 201, Salt Lake City, UT 84112 USA Univ Utah 30 S 2000 East,Room 201 Salt Lake City UT USA 84112
Citazione:
V. Sandoval et al., "Methamphetamine-induced rapid and reversible changes in dopamine transporter function: An in vitro model", J NEUROSC, 21(4), 2001, pp. 1413-1419

Abstract

This laboratory has demonstrated that a single methamphetamine (METH) injection rapidly and reversibly decreases the activity of the dopamine transporter (DAT), as assessed ex vivo in synaptosomes prepared from treated rats. This decrease does not occur because of residual drug introduced by the original injection or nor is it associated with a change in binding of the DAT ligand WIN35428. The purpose of this study was to elucidate the mechanismor mechanisms of this METH effect by determining whether direct application of this stimulant to synaptosomes causes changes in DAT similar to those observed ex vivo. Similar to the ex vivo effect, incubation of striatal synaptosomes with METH decreased DAT activity, but not WIN35428 binding: the effect on activity was not eliminated by repeated washing of synaptosomes. Also, as observed ex vivo, incubation with 3,4-methylenedioxymethamphetamine, but not cocaine or methylphenidate, caused a METH-like reduction in DAT function. The rapid and reversible METH-induced diminution in DAT activity did not occur because of a change in membrane potential, as assessed in vitro and ex vivo by [H-3]tetraphenylphosphonium accumulation. However, the METH-related decline in DAT function may be attributed to phosphorylation because NPC15437, a protein kinase C inhibitor, attenuated the METH-induced decline in DAT function. Similarities between previously reported effects ex vivo of a single METH injection on serotonin and norepinephrine transporter function and effects of direct METH application in vitro were also found. Together, these data demonstrate that the in vitro incubation model mimics the rapid and reversible effects observed after a single METH injection.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 18/01/20 alle ore 13:44:09