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Titolo:
Effect of acylglyceride content on the structure and function of reconstituted high density lipoprotein particles
Autore:
Braschi, S; Coffill, CR; Neville, TAM; Hutt, DM; Sparks, DL;
Indirizzi:
Univ Ottawa, Inst Heart, Lipoprot & Atherosclerosis Res Grp, Ottawa, ON K1Y 4W7, Canada Univ Ottawa Ottawa ON Canada K1Y 4W7 Res Grp, Ottawa, ON K1Y 4W7, Canada Univ Paris 12, F-94010 Creteil, France Univ Paris 12 Creteil France F-94010 v Paris 12, F-94010 Creteil, France Hop Henri Mondor, Serv Med Interne 5, F-94010 Creteil, France Hop Henri Mondor Creteil France F-94010 terne 5, F-94010 Creteil, France CHEO, Res Inst, Mol Genet Lab, Ottawa, ON K1H 8L1, Canada CHEO Ottawa ON Canada K1H 8L1 , Mol Genet Lab, Ottawa, ON K1H 8L1, Canada Ottawa Civic Hosp, Loeb Med Res Inst, Ottawa, ON K1Y 4E9, Canada Ottawa Civic Hosp Ottawa ON Canada K1Y 4E9 st, Ottawa, ON K1Y 4E9, Canada
Titolo Testata:
JOURNAL OF LIPID RESEARCH
fascicolo: 1, volume: 42, anno: 2001,
pagine: 79 - 87
SICI:
0022-2275(200101)42:1<79:EOACOT>2.0.ZU;2-R
Fonte:
ISI
Lingua:
ENG
Soggetto:
APOLIPOPROTEIN-A-I; LECITHIN-CHOLESTEROL ACYLTRANSFERASE; LPA-I; PLASMA-LIPOPROTEINS; TRANSFER PROTEIN; HEPATIC LIPASE; PRE-BETA; C-13 NMR; CONFORMATION; ACTIVATION;
Keywords:
HDL; apolipoprotein A-I; diglyceride; triglyceride; charge; stability; conformation; immunochemistry; LCAT;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
42
Recensione:
Indirizzi per estratti:
Indirizzo: Sparks, DL Univ Ottawa, Inst Heart, Lipoprot & Atherosclerosis Res Grp, Ottawa, ON K1Y 4W7, Canada Univ Ottawa Ottawa ON Canada K1Y 4W7 ttawa, ON K1Y 4W7, Canada
Citazione:
S. Braschi et al., "Effect of acylglyceride content on the structure and function of reconstituted high density lipoprotein particles", J LIPID RES, 42(1), 2001, pp. 79-87

Abstract

The effects of different acylglycerides on the conformation and charge of apolipoprotein A-I (apoA-I) have been investigated in reconstituted high density lipoproteins (LpA-I), Various amounts of diacylglycerol (DG) and triacylglycerol (TG) were incorporated into sonicated spherical LpA-I particlescontaining 2 molecules of apoA-I and 80 molecules of phospholipid, Inclusion of 30 molecules of TG into the LpA-I particle increases the net negativecharge of apoA-I (-8.5 to -9.3 mV), but has little effect on the amount and thermodynamic stability of the cr. helices in apoA-I. Incorporation of 30molecules of DG into the lipoprotein complex promotes a small increase in the cu-helix content and stability, but greatly increases the net negative charge of apoA-I (-8.5 to -11.2 mV), Inclusion of DG increases the immunoreactivity of two epitopes in the N terminus of apoA-I, but decreases the exposure of a domain closer to the C terminus (residues 148-186) of the apoprotein, In contrast, TG increases the exposure of epitopes over the entire apoA-I molecule; TG increases the immunoreactivity of epitopes for 13 different monoclonal antibodies to apoA-I, Incubations with purified lecithin:cholesterol acyltransferase show that cholesterol esterification is stimulated by DG, but inhibited by TG. The data show that TG and DG have different effects on apoA-I structure and function and this suggests that the TG-to-DG ratio in HDL may directly affect the metabolism of this lipoprotein class.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/04/20 alle ore 13:08:04