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Titolo:
Trangenic misexpression of the differentiation-specific desmocollin isoform 1 in basal keratinocytes
Autore:
Henkler, F; Strom, M; Mathers, K; Cordingley, H; Sullivan, K; King, I;
Indirizzi:
Natl Inst Med Res, Div Membrane Biol, London NW7 1AA, England Natl Inst Med Res London England NW7 1AA e Biol, London NW7 1AA, England
Titolo Testata:
JOURNAL OF INVESTIGATIVE DERMATOLOGY
fascicolo: 1, volume: 116, anno: 2001,
pagine: 144 - 149
SICI:
0022-202X(200101)116:1<144:TMOTDD>2.0.ZU;2-W
Fonte:
ISI
Lingua:
ENG
Soggetto:
DESMOSOMAL PLAQUE; EXPRESSION PATTERNS; EPIDERMIS; EPITHELIA; ADHESION; PROTEINS; FAMILY; MOUSE; CELLS; GENE;
Keywords:
cadherins; cell adhesion; desmosomes; keratinocytes;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
28
Recensione:
Indirizzi per estratti:
Indirizzo: King, I Natl Inst Med Res, Div Membrane Biol, The Ridgeway,Mill Hill, London NW7 1AA, England Natl Inst Med Res The Ridgeway,Mill Hill London England NW7 1AA d
Citazione:
F. Henkler et al., "Trangenic misexpression of the differentiation-specific desmocollin isoform 1 in basal keratinocytes", J INVES DER, 116(1), 2001, pp. 144-149

Abstract

Keratinocytes undergoing terminal differentiation are characterized by well-defined changes in protein expression, which contribute towards the transformation of cytoarchitecture and epithelial morphology, Characteristic patterns of desmosomal cadherins are tightly regulated and distinct isoforms are expressed during development and differentiation of epithelial tissues, Desmocollin-1 is strictly confined to suprabasal layers of epidermis, but it is absent in mitotically active, basal keratinocytes. This raises the question of whether basal desmocollin-1 could alter desmosomal functions and compromise keratinocyte proliferation, stratification, or early differentiation in skin. In this study, we misexpressed human desmocollin-1 in mouse epidermis, under control of the keratin-14 promoter. Transgenic animals were generated, which showed a specific expression of transgenic human desmocollin-1 in epidermal basal cells. High level transgenic expression, which was equal to or greater than endogenous protein levels, was observed in mice with multiple copy integration of the transgene. A punctate distribution of desmocollin-1 was demonstrated at the cell membrane by indirect immunofluorescence. Transgenic human desmocollin-1 colocalized with endogenous desmosomal marker proteins, indicating efficient incorporation into desmosomes. Transgenic mice did not display any obvious abnormalities, either in the histology of skin and hair follicles, or in the ultrastructure of desmosomes. These observations suggest that desmocollin-1 can function as a desmosomal cadherin both in basal and suprabasal cells. We propose that the differentiation-specific desmocollin isoforms desmocollin-1 and desmocollin-3 are functionally equivalent in basal epidermal cells and suggest that their changingexpression patterns are markers, but not regulators, of the initial steps in keratinocyte differentiation.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 13/07/20 alle ore 20:06:18