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Titolo:
Chromosomal aberrations characteristic for sAML/sMDS are not detectable byrandom screening using FISH in peripheral blood-derived grafts used for autologous transplantation
Autore:
Weber, MH; Wenzel, U; Thiel, E; Knauf, WU;
Indirizzi:
Free Univ Berlin, Klinikum Benjamin Franklin, Dept Med Haematol Oncol & Transfus Med 3, D-12200 Berlin, Germany Free Univ Berlin Berlin Germany D-12200 s Med 3, D-12200 Berlin, Germany
Titolo Testata:
JOURNAL OF HEMATOTHERAPY & STEM CELL RESEARCH
fascicolo: 6, volume: 9, anno: 2000,
pagine: 861 - 865
SICI:
1525-8165(200012)9:6<861:CACFSA>2.0.ZU;2-S
Fonte:
ISI
Lingua:
ENG
Soggetto:
BONE-MARROW TRANSPLANTATION; STEM-CELL TRANSPLANTATION; ACUTE NONLYMPHOCYTIC LEUKEMIA; MYELODYSPLASTIC SYNDROME; SECONDARY MYELODYSPLASIA; THERAPY; ABNORMALITIES; MALIGNANCIES; CHEMOTHERAPY; LYMPHOMA;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
19
Recensione:
Indirizzi per estratti:
Indirizzo: Knauf, WU Free Univ Berlin, Klinikum Benjamin Franklin, Dept Med Haematol Oncol & Transfus Med 3, Hindenburgdamm 30, D-12200 Berlin, Germany Free Univ Berlin Hindenburgdamm 30 Berlin Germany D-12200 rmany
Citazione:
M.H. Weber et al., "Chromosomal aberrations characteristic for sAML/sMDS are not detectable byrandom screening using FISH in peripheral blood-derived grafts used for autologous transplantation", J HEMATH ST, 9(6), 2000, pp. 861-865

Abstract

Acute myeloid leukemia (AML) or myelodysplastic syndrome (MDS) is observedin 5 to 10% of patients treated with high-dose chemotherapies followed by autologous stem cell and bone marrow transplantation. Both diseases are frequently associated with monosomy 7 (-7), trisomy 8 (+8), loss of the long arm of chromosome 5 (-5q), and deletions including the TP53-gene region according to del(17)(p13). In this study, we examined whether these chromosomalaberrations are already detectable in blood stem cells from patients who have all been treated with standard chemotherapies prior to peripheral bloodstem cell transplantation (PBSCT). Therefore, we screened peripheral bloodderived stem cells obtained at the time of stem cell harvest for the presence of -7, +8, -5q, and del(17)(p13) by fluorescence in situ hybridization (FISH). Our series included 40 patients: 4 patients with Hodgkin's disease,6 patients with non-Hodgkin-lymphoma (NHL), 1 patient with ALL, 4 patientswith plasmocytoma, and 25 patients with solid tumors. Peripheral blood mononuclear cells (PBMC) from eight healthy blood donors served as controls. Assuming a hybridization efficiency of >98%, the cut-off level of non diploid cells was determined for each DNA-probe. None of the stem cell preparations exhibited chromosomal damage. Our findings indicate that chromosomal damage is a rare event in stem cell autografts.

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Documento generato il 04/04/20 alle ore 08:50:43