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Titolo:
Cloning and characterization of cDNAs encoding the GnRH1 and GnRH2 precursors from bullfrog (Rana catesbeiana)
Autore:
Wang, L; Yoo, MS; Kang, HM; Im, WB; Choi, HS; Bogerd, J; Kwon, HB;
Indirizzi:
Chonnam Natl Univ, Hormone Res Ctr, Kwangju 500757, South Korea Chonnam Natl Univ Kwangju South Korea 500757 Kwangju 500757, South Korea Chonnam Natl Univ, Dept Biol, Kwangju 500757, South Korea Chonnam Natl Univ Kwangju South Korea 500757 Kwangju 500757, South Korea Univ Utrecht, Dept Expt Zool, Res Grp Comparat Endocrinol, NL-3508 TC Utrecht, Netherlands Univ Utrecht Utrecht Netherlands NL-3508 TC 3508 TC Utrecht, Netherlands
Titolo Testata:
JOURNAL OF EXPERIMENTAL ZOOLOGY
fascicolo: 3, volume: 289, anno: 2001,
pagine: 190 - 201
SICI:
0022-104X(20010215)289:3<190:CACOCE>2.0.ZU;2-P
Fonte:
ISI
Lingua:
ENG
Soggetto:
GONADOTROPIN-RELEASING-HORMONE; 2 MOLECULAR-FORMS; GENE-EXPRESSION; MESSENGER-RNA; NERVOUS-SYSTEM; 2ND GENE; BRAIN; FROG; LOCALIZATION; RIDIBUNDA;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
34
Recensione:
Indirizzi per estratti:
Indirizzo: Kwon, HB Chonnam Natl Univ, Hormone Res Ctr, Kwangju 500757, South Korea Chonnam Natl Univ Kwangju South Korea 500757 00757, South Korea
Citazione:
L. Wang et al., "Cloning and characterization of cDNAs encoding the GnRH1 and GnRH2 precursors from bullfrog (Rana catesbeiana)", J EXP ZOOL, 289(3), 2001, pp. 190-201

Abstract

We have isolated the cDNAs encoding the GnRH1 and GnRH2 precursors, respectively, from bullfrog (Rana catesbeiana) brain. The first cDNA consists of 648 bp and contains an open-reading frame of 270 nucleotides, encoding the bullfrog GnRH1 precursor. The second cDNA consists of 1053 bp and contains an open-reading frame of 255 nucleotides, encoding the bullfrog GnRH2 precursor. Both types of bullfrog GnRH precursor have a similar molecular architecture as observed in other GnRH precursors, consisting of a signal peptide, followed by the GnRH decapeptide, a conserved carboxy-terminal amidation and proteolytical processing site, and a GnRH-associated peptide (GAP). In addition, we have identified a third cDNA, containing 24 additional nucleotides in its GAP-coding region. Genomic PCR and sequence analysis confirmed that this cDNA represents an alternative splice variant of the bullfrog GnRH2-precursor pre-mRNA. The bullfrog GnRH1 precursor exhibits 60% and less than 40% amino acid identity to its Xenopus and mammalian counterparts, respectively, whereas the bullfrog GnRH2 precursor displays 50% to 60% amino acid identity to that of its nonmammalian counterparts, but shares only 25% amino acid identity with its mammalian counterparts. Northern blot analysis revealed a single GnRH1-precursor mRNA species of similar to0.75 kilobases, expressed in bullfrog forebrain, and a single GnRH2-precursor mRNA species of similar to1.1 kilobases, expressed in bullfrog midbrain/hindbrain. Furthermore, both bullfrog GnRH-precursor mRNAs exhibited a differential spatiotemporal expression pattern. Genomic Southern blot analysis indicated that both bullfrog GnRH genes are present as single copygenes. This is the first report on the molecular cloning of a GnRH2-precursor cDNA from an amphibian species. In addition, we present data showing that alternative splicing is utilized to generate different GnRH2-precursor mRNAs. (C) 2001 Wiley-Liss, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/12/20 alle ore 06:48:32