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Titolo:
Rapid detection of West Nile virus from human clinical specimens, field-collected mosquitoes, and avian samples by a TaqMan reverse transcriptase-PCRassay
Autore:
Lanciotti, RS; Kerst, AJ; Nasci, RS; Godsey, MS; Mitchell, CJ; Savage, HM; Komar, N; Panella, NA; Allen, BC; Volpe, KE; Davis, BS; Roehrig, JT;
Indirizzi:
CDC, Div Vector Borne Infect Dis, Natl Ctr Infect Dis, Ft Collins, CO 80521 USA CDC Ft Collins CO USA 80521 Natl Ctr Infect Dis, Ft Collins, CO 80521 USA
Titolo Testata:
JOURNAL OF CLINICAL MICROBIOLOGY
fascicolo: 11, volume: 38, anno: 2000,
pagine: 4066 - 4071
SICI:
0095-1137(200011)38:11<4066:RDOWNV>2.0.ZU;2-O
Fonte:
ISI
Lingua:
ENG
Soggetto:
ENCEPHALITIS; EPIDEMIC; ROMANIA; RNA;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
17
Recensione:
Indirizzi per estratti:
Indirizzo: Lanciotti, RS CDC, Div Vector Borne Infect Dis, Natl Ctr Infect Dis, Rampart Rd, Ft Collins, CO 80521 USA CDC Rampart Rd Ft Collins CO USA 80521 Collins, CO 80521 USA
Citazione:
R.S. Lanciotti et al., "Rapid detection of West Nile virus from human clinical specimens, field-collected mosquitoes, and avian samples by a TaqMan reverse transcriptase-PCRassay", J CLIN MICR, 38(11), 2000, pp. 4066-4071

Abstract

The authors report on the development and application of a rapid TaqMan assay for the detection of West Nile (WN) virus in a variety of human clinical specimens and field-collected specimens. Oligonucleotide primers and FAM-and TAMRA-labeled WN virus-specific probes were designed by using the nucleotide sequence of the New York 1999 WN virus isolate. The TaqMan assay wascompared to a traditional reverse transcriptase (RT)-PCR assay and to virus isolation in Vero cells with a large number (approximate to 5500) of specimens obtained from humans (serum, cerebrospinal fluid, and brain tissue), field-collected mosquitoes, and avian tissue samples. The TaqMan assay was specific for WN virus and demonstrated a greater sensitivity than the traditional RT-PCR method and correctly identified WN virus in 100% of the culture-positive mosquito pools and 98% of the culture-positive avian tissue samples. The assay should be of utility in the diagnostic laboratory to complement existing human diagnostic testing and as a tool to conduct WN virus surveillance in the United States.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/07/20 alle ore 22:13:23