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Titolo:
Transfusion-acquired, autochthonous human babesiosis in Japan: Isolation of Babesia microti-like parasites with hu-RBC-SCID mice
Autore:
Saito-Ito, A; Tsuji, M; Wei, Q; He, SY; Matsui, T; Kohsaki, M; Arai, S; Kamiyama, T; Hioki, K; Ishihara, C;
Indirizzi:
Rakuno Gakuen Univ, Sch Vet Med, Ebetsu, Hokkaido 0698501, Japan Rakuno Gakuen Univ Ebetsu Hokkaido Japan 0698501 Hokkaido 0698501, Japan Kobe Univ, Sch Med, Dept Med Zool, Kobe, Hyogo 6500017, Japan Kobe Univ Kobe Hyogo Japan 6500017 t Med Zool, Kobe, Hyogo 6500017, Japan Kobe Univ, Sch Med, Div 3, Dept Med, Kobe, Hyogo 6500017, Japan Kobe UnivKobe Hyogo Japan 6500017 , Dept Med, Kobe, Hyogo 6500017, Japan Hyogo Red Cross Blood Ctr, Kobe, Hyogo 6510062, Japan Hyogo Red Cross Blood Ctr Kobe Hyogo Japan 6510062 , Hyogo 6510062, Japan Natl Inst Infect Dis, Tokyo 1628640, Japan Natl Inst Infect Dis Tokyo Japan 1628640 nfect Dis, Tokyo 1628640, Japan Cent Inst Expt Anim, Kawasaki, Kanagawa 2160001, Japan Cent Inst Expt Anim Kawasaki Kanagawa Japan 2160001 nagawa 2160001, Japan
Titolo Testata:
JOURNAL OF CLINICAL MICROBIOLOGY
fascicolo: 12, volume: 38, anno: 2000,
pagine: 4511 - 4516
SICI:
0095-1137(200012)38:12<4511:TAHBIJ>2.0.ZU;2-3
Fonte:
ISI
Lingua:
ENG
Soggetto:
PLASMODIUM-FALCIPARUM; BORRELIA-BURGDORFERI; MOUSE MODEL; INFECTION; ERYTHROCYTES; CULTIVATION; PIROPLASM; DIVERGENS; ORGANISM;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
39
Recensione:
Indirizzi per estratti:
Indirizzo: Tsuji, M Rakuno Gakuen Univ, Sch Vet Med, 582-1 Bunkyodai midorimachi, Ebetsu, Hokkaido 0698501, Japan Rakuno Gakuen Univ 582-1 Bunkyodai midorimachiEbetsu Hokkaido Japan 0698501
Citazione:
A. Saito-Ito et al., "Transfusion-acquired, autochthonous human babesiosis in Japan: Isolation of Babesia microti-like parasites with hu-RBC-SCID mice", J CLIN MICR, 38(12), 2000, pp. 4511-4516

Abstract

We have isolated piroplasms from a patient who developed the first case ofhuman babesiosis in Japan by using NOD/shi-scid mice whose circulating erythrocytes (RBCs) had been replaced with human RBCs (hu-RBC-SCID mice). Following inoculation of the patients blood specimen into hu-RBC-SCID mice, parasites proliferated within the human RBCs in the mice, resulting in a high level of parasitemia. Parasite DNA was prepared from blood samples of the patient and the mice, and the nuclear small-subunit rRNA gene (rDNA) was amplified and sequenced. Both DNA samples gave rise to identical sequences,which shelved the highest degree of homology (99.2%) with the Babesia microti rDNA. Because the patient had received a blood transfusion before the onsetof babesiosis, we investigated the eight donors who were involved, Their archived blood samples were analyzed for specific antibody and parasite DNA;only a single donor was found to be positive by both tests, and the parasite rDNA sequence from the donor coincided with that derived from the patient, The donor's serum exihibited a high antibody titer against the isolate from the patient, whereas it exhibited only a weak cross-reaction against B.microti strains isolated in the United States. We conclude that the first Japanese babesiosis case occurred due to a blood transfusion and that the etiological agent is an indigenous Japanese parasite which may be a geographical variant of B. microti, Our results also demonstrated the usefulness ofhu-RBC-SCID mice for isolation of parasites from humans and for maintenance of the parasite infectivity for human RBCs.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 14/07/20 alle ore 09:39:49