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Titolo:
Analysis for a limited number of gene codons can predict drug resistance of Mycobacterium tuberculosis in a high-incidence community
Autore:
Van Rie, A; Warren, R; Mshanga, I; Jordaan, AM; van der Spuy, GD; Richardson, M; Simpson, J; Gie, RP; Enarson, DA; Beyers, N; van Helden, PD; Victor, TC;
Indirizzi:
Univ Stellenbosch, Dept Med Biochem, MRC, Ctr Cellular & Mol Biol, ZA-7600Stellenbosch, South Africa Univ Stellenbosch Stellenbosch South Africa ZA-7600 nbosch, South Africa Univ Stellenbosch, Dept Pediat & Child Hlth, ZA-7600 Stellenbosch, South Africa Univ Stellenbosch Stellenbosch South Africa ZA-7600 nbosch, South Africa S African Inst Med Res, Cape Town, South Africa S African Inst Med Res Cape Town South Africa , Cape Town, South Africa Fac Med, Muhimbili Med Ctr, Dept Biochem, Dar Es Salaam, Tanzania Fac MedDar Es Salaam Tanzania r, Dept Biochem, Dar Es Salaam, Tanzania Int Union TB & Lung Dis, Paris, France Int Union TB & Lung Dis Paris France Union TB & Lung Dis, Paris, France
Titolo Testata:
JOURNAL OF CLINICAL MICROBIOLOGY
fascicolo: 2, volume: 39, anno: 2001,
pagine: 636 - 641
SICI:
0095-1137(200102)39:2<636:AFALNO>2.0.ZU;2-5
Fonte:
ISI
Lingua:
ENG
Soggetto:
NEW-YORK-CITY; MUTATIONS; RIFAMPIN; TRANSMISSION; STREPTOMYCIN; STRAINS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
25
Recensione:
Indirizzi per estratti:
Indirizzo: Victor, TC Univ Stellenbosch, Dept Med Biochem, MRC, Ctr Cellular & Mol Biol, ZA-7600Stellenbosch, South Africa Univ Stellenbosch Stellenbosch South Africa ZA-7600 th Africa
Citazione:
A. Van Rie et al., "Analysis for a limited number of gene codons can predict drug resistance of Mycobacterium tuberculosis in a high-incidence community", J CLIN MICR, 39(2), 2001, pp. 636-641

Abstract

Correct and rapid diagnosis is essential in the management of multidrug-resistant tuberculosis (MDR-TB). In this population-based study of 61 patients with drug-resistant tuberculosis, we evaluated the frequency of mutationsand compared the performance of genotypic (mutation analysis by dot blot hybridization) and phenotypic (indirect proportion method) drug resistance tests. Three selected codons (rpoB531, rpoB526, and katG315) allowed identification of 90% of MDR-TB cases. Ninety percent of rifampin, streptomycin, and ethambutol resistance and 75% of isoniazid resistance were detected by screening for six codons: rpoB531, rpoB526, rrs-513, rpsL43, embB306, and katG315. The performance (reproducibility, sensitivity, and specificity) of the genotypic method was superior to that of the routine phenotypic method, with the exception of sensitivity for isoniazid resistance. A commercialized molecular genetic test for a limited number of target loci might be a good alternative for a drug resistance screening test in the context of an MDR"DOTS-plus" strategy.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/03/20 alle ore 19:03:00