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Titolo:
Dynamin is involved in human epithelial cell vacuolation caused by the Helicobacter pylori-produced cytotoxin VacA
Autore:
Suzuki, J; Ohnsihi, H; Shibata, H; Wada, A; Hirayama, T; Iiri, T; Ueda, N; Kanamaru, C; Tsuchida, T; Mashima, H; Yasuda, H; Fujita, T;
Indirizzi:
Univ Tokyo, Sch Med, Dept Internal Med, Bunkyo Ku, Tokyo 1128688, Japan Univ Tokyo Tokyo Japan 1128688 rnal Med, Bunkyo Ku, Tokyo 1128688, Japan Gunma Univ, Dept Cell Biol Mol & Cellular Regulat, Maebashi, Gumma 371, Japan Gunma Univ Maebashi Gumma Japan 371 r Regulat, Maebashi, Gumma 371, Japan Nagasaki Univ, Inst Trop Med, Dept Bacteriol, Nagasaki 852, Japan NagasakiUniv Nagasaki Japan 852 ed, Dept Bacteriol, Nagasaki 852, Japan
Titolo Testata:
JOURNAL OF CLINICAL INVESTIGATION
fascicolo: 3, volume: 107, anno: 2001,
pagine: 363 - 370
SICI:
0021-9738(200102)107:3<363:DIIIHE>2.0.ZU;2-K
Fonte:
ISI
Lingua:
ENG
Soggetto:
VESICLE FORMATION; MOLECULAR MOTOR; MUTANT DYNAMIN; IN-VITRO; PROTEIN; TRANSPORT; BINDING; LINES;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
27
Recensione:
Indirizzi per estratti:
Indirizzo: Ohnsihi, H Univ Tokyo, Sch Med, Dept Internal Med, Bunkyo Ku, 3-28-6 Mejirodai, Tokyo1128688, Japan Univ Tokyo 3-28-6 Mejirodai Tokyo Japan 1128688 1128688, Japan
Citazione:
J. Suzuki et al., "Dynamin is involved in human epithelial cell vacuolation caused by the Helicobacter pylori-produced cytotoxin VacA", J CLIN INV, 107(3), 2001, pp. 363-370

Abstract

The Helicobacter pylori-produced cytotoxin VacA induces intracellular vacuolation. To elucidate the molecular mechanism of vacuole formation by VacA,we examined the participation of dynamin, a GTPase functioning in intracellular vesicle formation, in human HeLa cells. Immunocytochemistry revealed that endogenous dynamin was localized to vacuoles induced by VacA, In cellstransiently transfected with a GTPase-defective (dominant-negative) dynamin mutant, VacA Failed to induce vacuolation. In contrast, VacA did induce vacuolation in cells transiently transfected with wild-type dynamin, Furthermore, under VacA treatment, neutral red dye uptake, a parameter of VacA-induced vacuolation, was inhibited in cells stably transfected with the dominant-negative dynamin mutant. In contrast, uptake was markedly enhanced in cells stably transfected with wild-type dynamin. Moreover, VacA cytopathic effects on the viability of HeLa cells were inhibited in cells stably transfected with dominant-negative dynamin-1. Sequential immunocytochemical observation confirmed that expression of dominant-negative dynamin did not affectVacA attachment to or internalization into HeLa cells. We suggest that dynamin is involved in the intracellular vacuolation induced by VacA.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 17/02/20 alle ore 17:18:40