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Titolo:
Column-switching high-performance liquid chromatography-electrospray ionization mass spectrometry for identification of heroin metabolites in human urine
Autore:
Katagi, M; Nishikawa, M; Tatsuno, M; Miki, A; Tsuchihashi, H;
Indirizzi:
Forens Sci Lab, Chuo Ku, Osaka 5410053, Japan Forens Sci Lab Osaka Japan5410053 ci Lab, Chuo Ku, Osaka 5410053, Japan
Titolo Testata:
JOURNAL OF CHROMATOGRAPHY B
fascicolo: 1, volume: 751, anno: 2001,
pagine: 177 - 185
SICI:
1387-2273(20010210)751:1<177:CHLCI>2.0.ZU;2-O
Fonte:
ISI
Lingua:
ENG
Soggetto:
GAS-CHROMATOGRAPHY; CLINICAL TOXICOLOGY; BIOLOGICAL-FLUIDS; SOLID-PHASE; HUMAN SERUM; GC-MS; MORPHINE; CODEINE; 6-MONOACETYLMORPHINE; 6-ACETYLMORPHINE;
Keywords:
heroin; column-switching; HPLC-ESI-MS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
36
Recensione:
Indirizzi per estratti:
Indirizzo: Katagi, M Forens Sci Lab, Chuo Ku, Osaka Prefectural Police HQ,1-3-18 Hommachi, Osaka 5410053, Japan Forens Sci Lab Osaka Prefectural Police HQ,1-3-18 Hommachi Osaka Japan 5410053
Citazione:
M. Katagi et al., "Column-switching high-performance liquid chromatography-electrospray ionization mass spectrometry for identification of heroin metabolites in human urine", J CHROMAT B, 751(1), 2001, pp. 177-185

Abstract

In order to prove heroin (DAM) use, a simple, rapid and sensitive analytical method has been established by combining semi-microcolumn HPLC, a columnswitching technique and electrospray ionization mass spectrometry (ESI-MS). Urine samples were directly introduced to the system, and endogenous urinary constituents were removed by using on-line column switching solid-phaseextraction with a strong cation-exchange (SCX) cartridge column (2.0 mm I.D. X 10 mm). Heroin and its metabolites enriched on the top of the column were then successfully analyzed with excellent separation by use of a SCX semi-microcolumn (1.5 mm I.D. X 150 mm), accompanied by ESI mass spectral detection. The proposed conditions are as follows: mobile phase, 10 mM ammonium acetate (pH 6.0)-acetonitrile (30:70, v/v) (for main separation) and 30 mM ammonium acetate (for trapping); flow-rates, 120 mul/min (for main separation) and 200 mul/min (for trapping); capillary voltage, +4.5 kV; cone voltage, 50 V. Linear calibration curves were obtained in the selected ion monitoring (SLM) mode using protonated molecular ions (m/z 370 for DAM, m/z 328for MAM and mit 286 for MOR) over the concentration ranges from 10 to 1000ng/ml for morphine (MOR) and 1-100 ng/ml for DAM and 6-acetylmorphine (MAM). The detection limits were 0.1-3 ng/ml. Upon applying the scan mode, 2-30ng/ml were the detection limits. The present HPLC-ESI-MS method was successfully applied to the determination of opiates in users' urine samples. (C)2001 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/04/20 alle ore 08:13:51