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Titolo:
Sedimentation field-flow fractionation device cleaning, decontamination and sterilization procedures for cellular analysis
Autore:
Battu, S; Roux, A; Delebasee, S; Bosgiraud, C; Cardot, PJP;
Indirizzi:
Univ Limoges, Fac Pharm, Lab Chim Analyt & Bromatol, F-87025 Limoges, France Univ Limoges Limoges France F-87025 & Bromatol, F-87025 Limoges, France Univ Limoges, Fac Pharm, Lab Bacteriol Virol & Immunol, F-87025 Limoges, France Univ Limoges Limoges France F-87025 l & Immunol, F-87025 Limoges, France
Titolo Testata:
JOURNAL OF CHROMATOGRAPHY B
fascicolo: 1, volume: 751, anno: 2001,
pagine: 131 - 141
SICI:
1387-2273(20010210)751:1<131:SFFDCD>2.0.ZU;2-V
Fonte:
ISI
Lingua:
ENG
Soggetto:
RED-BLOOD-CELLS; PHYSICAL CHARACTERIZATION; STAPHYLOCOCCUS-AUREUS; SEPARATION; BACTERIA; ELUTION; QUANTIFICATION; PURIFICATION; RETENTION; WALL;
Keywords:
sedimentation field-flow fractionation; cleaning; sterilization; cellular analysis;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
39
Recensione:
Indirizzi per estratti:
Indirizzo: Battu, S Univ Limoges, Fac Pharm, Lab Chim Analyt & Bromatol, 2 Rue Docteur Marcland, F-87025 Limoges, France Univ Limoges 2 Rue Docteur Marcland Limoges France F-87025 rance
Citazione:
S. Battu et al., "Sedimentation field-flow fractionation device cleaning, decontamination and sterilization procedures for cellular analysis", J CHROMAT B, 751(1), 2001, pp. 131-141

Abstract

In Sedimentation FFF (SdFFF) practice, it is known that a large number of cell elutions create aging phenomena of the separator, thereby reducing recovery and modifying elution characteristics. Systematic cleaning proceduresare developed to enhance channel lifetime, together with microbial decontamination processes. Cells can be therefore reproducibly eluted for a large number of analyses and collected under sterile conditions, if needed. This is one of the most valuable aspect if further culture or transplantation isrequired. Decontamination was performed using, as contaminant probe, Staphylococcus aureus, highly adherent pathogenic bacteria that eluted from SdFFF as aggregates. (C) 2001 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 25/01/20 alle ore 16:23:43