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Titolo:
Properties of a stationary phase based on immobilised chicken liver basic fatty acid-binding protein
Autore:
Massolini, G; De Lorenzi, E; Calleri, E; Bertucci, C; Monaco, HL; Perduca, M; Caccialanza, G; Wainer, IW;
Indirizzi:
Univ Pavia, Dept Pharmaceut Chem, I-27100 Pavia, Italy Univ Pavia Pavia Italy I-27100 ept Pharmaceut Chem, I-27100 Pavia, Italy Univ Bologna, Dept Pharmaceut Sci, I-40126 Bologna, Italy Univ Bologna Bologna Italy I-40126 harmaceut Sci, I-40126 Bologna, Italy Univ Pavia, Dept Genet, I-27100 Pavia, Italy Univ Pavia Pavia Italy I-27100 v Pavia, Dept Genet, I-27100 Pavia, Italy Univ Verona, Dept Sci & Technol, I-37134 Verona, Italy Univ Verona Verona Italy I-37134 pt Sci & Technol, I-37134 Verona, Italy Georgetown Univ, Med Ctr, Dept Pharmacol, Washington, DC 20007 USA Georgetown Univ Washington DC USA 20007 armacol, Washington, DC 20007 USA
Titolo Testata:
JOURNAL OF CHROMATOGRAPHY B
fascicolo: 1, volume: 751, anno: 2001,
pagine: 117 - 130
SICI:
1387-2273(20010210)751:1<117:POASPB>2.0.ZU;2-Y
Fonte:
ISI
Lingua:
ENG
Soggetto:
PERFORMANCE LIQUID-CHROMATOGRAPHY; STRUCTURE-RETENTION RELATIONSHIPS; HUMAN SERUM-ALBUMIN; AFFINITY-CHROMATOGRAPHY; REVERSED-PHASE; SEPARATION; RESOLUTION; DRUGS; VIEW;
Keywords:
chiral stationary phases; LC; proteins; fatty acids;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
16
Recensione:
Indirizzi per estratti:
Indirizzo: Massolini, G Univ Pavia, Dept Pharmaceut Chem, Via Taramelli 12, I-27100 Pavia, Italy Univ Pavia Via Taramelli 12 Pavia Italy I-27100 Pavia, Italy
Citazione:
G. Massolini et al., "Properties of a stationary phase based on immobilised chicken liver basic fatty acid-binding protein", J CHROMAT B, 751(1), 2001, pp. 117-130

Abstract

The fatty acid-binding proteins (FABPs) are a class of low-molecular-mass proteins that bind fatty acids and are thought to be involved in their intracellular transport. FABPs have been isolated and studied from several tissues, but their precise function and mechanism of action are still, not clear. Chicken liver (basic) fatty acid-binding protein (bFABP) was immobilisedon aminopropyl silica and the developed stationary phase was used to examine the enantioselective properties of this protein and to study the bindingof drugs to bFABP The retention and enantioselectivity of the new column for a large number of chiral drugs was investigated. The enantiomers of basic and neutral compounds were poorly retained and not resolved by the bFABP column. On the contrary the resolution of the enantiomers of some acidic compounds was obtained. Therefore the influence of the mobile phase pH and organic modifier on the chromatographic performance of acidic compounds was studied. In order to clarify the retention mechanism. competitive displacement studies were also carried out by adding short-chain fatty acids to the mobile phase as displacing agents and preliminary quantitative structure-retention relationship correlations were developed to describe the nature of the interactions between the chemical structures of the analytes and the observed chromatographic results. (C) 2001 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/04/20 alle ore 07:08:17