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Titolo:
Determination of terbutaline enantiomers in human urine by coupled achiral-chiral high-performance liquid chromatography with fluorescence detection
Autore:
Kim, KH; Kim, HJ; Kim, JH; Shin, SD;
Indirizzi:
Kangweon Natl Univ, Coll Pharm, Chunchon 200701, South Korea Kangweon NatlUniv Chunchon South Korea 200701 nchon 200701, South Korea
Titolo Testata:
JOURNAL OF CHROMATOGRAPHY B
fascicolo: 1, volume: 751, anno: 2001,
pagine: 69 - 77
SICI:
1387-2273(20010210)751:1<69:DOTEIH>2.0.ZU;2-O
Fonte:
ISI
Lingua:
ENG
Soggetto:
CAPILLARY ELECTROPHORESIS; COLUMN CHROMATOGRAPHY; BIOLOGICAL SAMPLES; MASS-SPECTROMETRY; DRUG ENANTIOMERS; SEPARATION; PLASMA; METABOLITE;
Keywords:
enantiomer separation; terbutaline;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
15
Recensione:
Indirizzi per estratti:
Indirizzo: Kim, KH Kangweon Natl Univ, Coll Pharm, Chunchon 200701, South Korea Kangweon Natl Univ Chunchon South Korea 200701 0701, South Korea
Citazione:
K.H. Kim et al., "Determination of terbutaline enantiomers in human urine by coupled achiral-chiral high-performance liquid chromatography with fluorescence detection", J CHROMAT B, 751(1), 2001, pp. 69-77

Abstract

A coupled achiral-chiral high-performance liquid chromatographic system with fluorescence detection at excitation! emission wavelengths of 276/306 nmhas been developed for the determination of the enantiomers of terbutaline, (S)-(+)-terbutaline and (R)-(-)-terbutaline in urine. Urine samples were prepared by solid-phase extraction with Sep-pak silica, followed by HPLC. The terbutaline was preseparated from the interfering components in urine onPhenomenex silica column and the terbutaline enantiomers and betaxolol were resolved and determined on a Sumichiral OA-4900 chiral stationary phase. The two columns were connected by a switching valve equipped with silica precolumn. The precolumn was used to concentrate the terbutaline in the eluent from the achiral column before back flushing onto the chiral phase. For each enantiomer the assay was linear between 1 and 250 ng/ml (R-2=0.9999) and the detection limit was 0.3 ng/ml. The intra-day variation was between 4.6 and 11.6% in relation to the measured concentration and the inter-day variation was 4.3-11.0%. It has been applied to the determination of (S)-(+)-terbutaline and (R)-(-)-terbutaline in urine from a healthy volunteer dosed with racemic terbutaline sulfate. (C) 2001 Elsevier Science B.V. All rightsreserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/04/20 alle ore 06:12:23