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Titolo:
Stereospecific analysis of ecstasy-like N-ethyl-3,4-ethylenedioxyamphetamine and its metabolites in humans
Autore:
Brunnenberg, M; Kovar, KA;
Indirizzi:
Dept Pharmaceut Chem, D-72076 Tubingen, Germany Dept Pharmaceut Chem Tubingen Germany D-72076 D-72076 Tubingen, Germany
Titolo Testata:
JOURNAL OF CHROMATOGRAPHY B
fascicolo: 1, volume: 751, anno: 2001,
pagine: 9 - 18
SICI:
1387-2273(20010210)751:1<9:SAOEN>2.0.ZU;2-Z
Fonte:
ISI
Lingua:
ENG
Soggetto:
3,4-METHYLENEDIOXYETHYLAMPHETAMINE MDE; 3,4-METHYLENEDIOXYMETHAMPHETAMINE; ELECTROPHORESIS; CYCLODEXTRIN; URINE; MDMA; RAT;
Keywords:
ecstasy; N-ethyl-3,4-methylenedioxyamphetamine;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
19
Recensione:
Indirizzi per estratti:
Indirizzo: Kovar, KA Dept Pharmaceut Chem, Morgenstelle 8, D-72076 Tubingen, Germany Dept Pharmaceut Chem Morgenstelle 8 Tubingen Germany D-72076 ny
Citazione:
M. Brunnenberg e K.A. Kovar, "Stereospecific analysis of ecstasy-like N-ethyl-3,4-ethylenedioxyamphetamine and its metabolites in humans", J CHROMAT B, 751(1), 2001, pp. 9-18

Abstract

A chiral HPLC method has been developed for the ecstasy analogue (R,S)-N-ethyl-3,4-methylenedioxyamphetamine (MDE) and its metabolites o-glucuronyl-(R,S)-N-ethyl-4-hydroxy-3-methoxyamphetamine (HME) and (R,S)-3,4-methylenedioxyamphetamine (MDA) in human plasma. The chiral discrimination of the compounds was carried out with an enantioselective HPLC method using beta -cyclodextrin in the mobile phase for MDE and MDA and a chiral protein phase (chiral-CBH) for HME. MDE and MDA were detected fluorimetrically at 322 nm, while the major metabolite HME was selectively determined by electrochemical detection at +600 mV. After hydrolysis of the conjugates using beta -glucuronidase/arylsulfatase and solid-phase extraction with a cation-exchange phase for sample preparation high recovery rates of more than 95% were yielded. The limit of quantitation for the enantiomers of MDE and its metabolites in plasma were between 1.2 (MDA) and 16 ng/ml(HME) and the relative method standard deviations (V-x0, Table 1) were less than 3%. The methods described have been used successfully in the enantioselective quantitation of the compounds in plasma samples obtained from six healthy volunteers in a clinical study after oral administration of 140 mg racemic MDE hydrochloride. Significant differences were found in the plasma concentrations of the examined stereoisomers. Whereas the R-enantiomer of the parent substance, MDE, waspredominant in the plasma samples investigated, higher plasma concentrations of the S-enantiomers of the metabolites MDA and HME were measured. (C) 2001 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/01/20 alle ore 21:38:11