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Titolo:
CUC-initiated FCF-2 induces chromatin compaction in cultured cardiac myocytes and in vitro
Autore:
Sun, CP; Doble, BW; Sun, JM; Fandrich, RR; Florkiewicz, R; Kirshenbaum, L; Davie, JR; Cattini, PA; Kardami, E;
Indirizzi:
Univ Manitoba, Fac Med, Dept Human Anat & Cell Biol, Winnipeg, MB, Canada Univ Manitoba Winnipeg MB Canada Anat & Cell Biol, Winnipeg, MB, Canada Univ Manitoba, Fac Med, Dept Physiol, Winnipeg, MB, Canada Univ Manitoba Winnipeg MB Canada Med, Dept Physiol, Winnipeg, MB, Canada Univ Manitoba, Fac Med, Manitoba Inst Cell Biol, Winnipeg, MB, Canada UnivManitoba Winnipeg MB Canada ba Inst Cell Biol, Winnipeg, MB, Canada Ciblex Corp, San Diego, CA USA Ciblex Corp San Diego CA USACiblex Corp, San Diego, CA USA
Titolo Testata:
JOURNAL OF CELLULAR PHYSIOLOGY
fascicolo: 3, volume: 186, anno: 2001,
pagine: 457 - 467
SICI:
0021-9541(200103)186:3<457:CFICCI>2.0.ZU;2-4
Fonte:
ISI
Lingua:
ENG
Soggetto:
FIBROBLAST-GROWTH-FACTOR; PROGRAMMED CELL-DEATH; MOLECULAR-WEIGHT FORMS; FACTOR-II; FACTOR BFGF; CHROMOSOME CONDENSATION; NH2-TERMINAL EXTENSION; NUCLEAR-LOCALIZATION; MOUSE FIBROBLASTS; HISTONE H3;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
48
Recensione:
Indirizzi per estratti:
Indirizzo: Kardami, E Univ Manitoba, Inst Cardiovasc Sci, SBGH Res Ctr 3008, 351 Tache Ave, Winnipeg, MB R2H 2A6, Canada Univ Manitoba 351 Tache Ave Winnipeg MBCanada R2H 2A6 , Canada
Citazione:
C.P. Sun et al., "CUC-initiated FCF-2 induces chromatin compaction in cultured cardiac myocytes and in vitro", J CELL PHYS, 186(3), 2001, pp. 457-467

Abstract

Fibroblast growth factor-2 (FGF-2) is a mitogen found in CUC-initiated 21-25 kDa ("hi") or AUG-initiated 16-18 kDa ("lo") forms. Previously we demonstrated that "hi"-but not "lo"-FGF-2 caused a distinct nuclear phenotype characterized by apparently condensed chromatin present as separate clumps in the nucleus of cardiac myocytes. In this manuscript we investigated whetherthese effects were related to apoptosis or mitosis and whether they reflected a direct effect of "hi" FGF-2 on chromatin. Myocytes overexpressing "hi" FGF-2 and presenting the clumped chromatin phenotype: (i) were not labeled above background with antibodies to phosphorylated histones H1 and H3 used as indicators of mitotic chromatin condensation; (ii) did not stain positive for TUNEL; (iii) their nuclear lamina, visualized by anti-laminB immunofluorescence, appeared intact; (iv) neither caspase inhibitors, nor Bcl-2 or "lo" FGF-2 overexpression prevented the manifestation of the compacted nuclear phenotype. Purified recombinant "hi" FGF-2 was more potent than "lo" FGF-2 in promoting the condensation/aggregation of chick erythrocyte chromatin partially reconstituted with histone H1 in vitro. We conclude that the DNA phenotype induced by "hi" FGF-2 in cardiac myocytes likely reflects a direct effect on chromatin structure that does not require the engagement ofmitosis or apoptosis. By affecting chromatin compaction "hi" FGF-2 may contribute to the regulation of gene expression. (C) 2001 Wiley-Liss, Inc.

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Documento generato il 25/09/20 alle ore 00:10:59