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Titolo:
An endoplasmic reticulum-specific stress-activated caspase (caspase-12) isimplicated in the apoptosis of A549 epithelial cells by respiratory syncytial virus
Autore:
Bitko, V; Barik, S;
Indirizzi:
Univ S Alabama, Coll Med, Dept Biochem & Mol Biol, Mobile, AL 36688 USA Univ S Alabama Mobile AL USA 36688 ochem & Mol Biol, Mobile, AL 36688 USA
Titolo Testata:
JOURNAL OF CELLULAR BIOCHEMISTRY
fascicolo: 3, volume: 80, anno: 2001,
pagine: 441 - 454
SICI:
0730-2312(2001)80:3<441:AERSC(>2.0.ZU;2-S
Fonte:
ISI
Lingua:
ENG
Soggetto:
NF-KAPPA-B; TRANSCRIPTION FACTOR; GENE-EXPRESSION; DNA FRAGMENTATION; NUCLEAR TRANSLOCATION; REQUIRES ACTIVATION; INTERLEUKIN-8 GENE; MEDIATED APOPTOSIS; SODIUM-SALICYLATE; IMMUNE-RESPONSE;
Keywords:
apoptosis; caspase; endoplasmic reticulum; fas; NF-kappa B; respiratory syncytial virus;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
58
Recensione:
Indirizzi per estratti:
Indirizzo: Barik, S Univ S Alabama, Coll Med, Dept Biochem & Mol Biol, 307 Univ BLvd,MSB 2370,Mobile, AL 36688 USA Univ S Alabama 307 Univ BLvd,MSB 2370 Mobile AL USA 36688 688 USA
Citazione:
V. Bitko e S. Barik, "An endoplasmic reticulum-specific stress-activated caspase (caspase-12) isimplicated in the apoptosis of A549 epithelial cells by respiratory syncytial virus", J CELL BIOC, 80(3), 2001, pp. 441-454

Abstract

Respiratory syncytial virus (RSV) infection induced programmed cell death or apoptosis in the cultured lung epithelial cell line, A549. The apoptoticcells underwent multiple changes, including fragmentation and degradation of genomic DNA, consistent with the activation of the DNA fragmentation factor or caspase-activated DNase (DFF or CAD). The infection led to activation of FasL; however, a transdominant mutant of FAS-downstream death domain protein, FADD, did not inhibit apoptosis. Similarly, modest activation of cytoplasmic apoptotic caspases, caspase-3 and -8, were observed; however, only a specific inhibitor of caspases-3 inhibited apoptosis, while an inhibitor of caspase-8 had little effect. No activation of caspase-9 and -10, indicators of the mitochondrial apoptotic pathway, was observed. In contrast, RSV infection strongly activated caspase-12, an endoplasmic reticulum (ER) stress response caspase. Activation of the ER stress response was further evidenced by upregulation of ER chaperones BiP and calnexin. Antisense-mediated inhibition of caspase-12 inhibited apoptosis. Inhibitors of NF-kappa B had no effect on apoptosis. Thus, RSV-induced apoptosis appears to occur through an ER stress response that activates caspase-12, and is uncoupled from NF-kappa B activation. (C) 2001 Wiley-Liss, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/09/20 alle ore 22:39:41