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Titolo:
Activation of extracellular signal-regulated kinases (ERKs) defines the first phase of 1,25-dihydroxyvitamin D-3-induced differentiation of HL60 cells
Autore:
Wang, XN; Studzinski, GP;
Indirizzi:
Univ Med & Dent New Jersey, New Jersey Med Sch, Dept Lab Med & Pathol, Newark, NJ 07103 USA Univ Med & Dent New Jersey Newark NJ USA 07103 thol, Newark, NJ 07103 USA
Titolo Testata:
JOURNAL OF CELLULAR BIOCHEMISTRY
fascicolo: 4, volume: 80, anno: 2001,
pagine: 471 - 482
SICI:
0730-2312(2001)80:4<471:AOESK(>2.0.ZU;2-U
Fonte:
ISI
Lingua:
ENG
Soggetto:
NERVE GROWTH-FACTOR; PROTEIN-KINASE; HL-60 CELLS; MONOCYTIC DIFFERENTIATION; SUSTAINED ACTIVATION; MEGAKARYOCYTIC DIFFERENTIATION; PROMYELOCYTIC LEUKEMIA; MAMMALIAN-CELLS; CYCLIN D1; MAP;
Keywords:
differentiation; signal transduction; vitamin D; leukemic cells; cell cycle; p27/Kip1;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
42
Recensione:
Indirizzi per estratti:
Indirizzo: Studzinski, GP Univ Med & Dent New Jersey, New Jersey Med Sch, Dept Lab Med & Pathol, 185S Orange Ave, Newark, NJ 07103 USA Univ Med & Dent New Jersey 185 S Orange Ave Newark NJ USA 07103
Citazione:
X.N. Wang e G.P. Studzinski, "Activation of extracellular signal-regulated kinases (ERKs) defines the first phase of 1,25-dihydroxyvitamin D-3-induced differentiation of HL60 cells", J CELL BIOC, 80(4), 2001, pp. 471-482

Abstract

Activation of ERK1 and ERK2 protein kinases has been implicated in diversecellular processes, including the control of cell proliferation and cell differentiation (Marshal[ [1995] Cell 80:179). In human myeloblastoid leukemia HL60 cells rapid (ca. 15 min) but transient activation of ERK1/2 has been reported following induction of macrophageimonocyle differentiation by phorbol esters, or by very high (10(-6) M) concentrations of 1,25-dihydroxyvitamin D-3 (1,25D(3)), while retinoic acid-induced granulocytic differentiation was accompanied by sustained activation of ERK1/2. We report here that monocytic differentiation of HL60 cells induced by moderate (10(-9) to 10(-7) M) concentrations of 1,25D(3) could be divided into at least two stages.in the first phase, which lasts 24-48 h, the cells continued in the normalcell cycle while expressing markers of monocytic phenotype, such as CD14. In the next phase the onset of G1 cell cycle block became apparent and expression of CD11b was prominent, indicating a more mature myeloid phenotype. The first phase was characterized by high levels of ERKs activated by phosphorylation, and these decreased as the cells entered the second phase, while the levers of p27/Kip1 increased at that time. Serum-starved or PD98059-treated HL60 cells had reduced growth rate and slower differentiation, but the G1 block also coincided with decreased levels of activated ERK1/2. The data suggest that the MEK/ERK pathway maintains cell proliferation during 1,25D(3)-induced monocytic differentiation of HL60 cells, but that ERK1/2 activity becomes suppressed during the rater stages of differentiation, and the consequent G1 block leads to "terminal" differentiation. J. Cell. Biochem. 80:471-482, 2001. (C) 2001 Wiley-Liss, Inc.

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Documento generato il 01/12/20 alle ore 08:09:17