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Titolo:
A calcineurin- and NFAT-dependent pathway regulates Myf5 gene expression in skeletal muscle reserve cells
Autore:
Friday, BB; Pavlath, GK;
Indirizzi:
Emory Univ, Sch Med, Dept Pharmacol, Atlanta, GA 30322 USA Emory Univ Atlanta GA USA 30322 ed, Dept Pharmacol, Atlanta, GA 30322 USA
Titolo Testata:
JOURNAL OF CELL SCIENCE
fascicolo: 2, volume: 114, anno: 2001,
pagine: 303 - 310
SICI:
0021-9533(200101)114:2<303:ACANPR>2.0.ZU;2-S
Fonte:
ISI
Lingua:
ENG
Soggetto:
CYCLOSPORINE-A; TRANSCRIPTION FACTORS; MOUSE MYOGENESIS; TRANSGENIC MICE; UP-REGULATION; MYOD FAMILY; FIBER-TYPE; MRF4; DIFFERENTIATION; HYPERTROPHY;
Keywords:
calcineurin; NFAT; gene regulation;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
47
Recensione:
Indirizzi per estratti:
Indirizzo: Pavlath, GK Emory Univ, Sch Med, Dept Pharmacol, Atlanta, GA 30322 USA Emory Univ Atlanta GA USA 30322 rmacol, Atlanta, GA 30322 USA
Citazione:
B.B. Friday e G.K. Pavlath, "A calcineurin- and NFAT-dependent pathway regulates Myf5 gene expression in skeletal muscle reserve cells", J CELL SCI, 114(2), 2001, pp. 303-310

Abstract

Myf5 is a member of the muscle regulatory factor family of transcription factors and plays an important role in the determination, development, and differentiation of skeletal muscle. However, factors that regulate the expression and activity of Myf5 itself are not well understood. Recently, a rolefor the calcium-dependent phosphatase calcineurin was suggested in three distinct pathways in skeletal muscle: differentiation, hypertrophy, and fiber-type determination, We propose that one downstream target of calcineurin and the calcineurin substrate NFAT in skeletal muscle is regulation of Myf5gene expression, For these studies, we used myotube cultures that contain both multinucleated myotubes and quiescent, mononucleated cells termed 'reserve' cells, which share many characteristics with satellite cells. Treatment of such myotube cultures,vith the calcium ionophore ionomycin results inan approximate to4-fold increase in Myf5 mRNA levels, but similar effects are not observed in proliferating myoblast cultures indicating that Myf5 isregulated by different pathways in different cell populations. The increase in Myf5 mRNA levels in myotube cultures requires the activity of calcineurin and NFAT, and can be specifically enhanced by overexpressing the NFATc isoform, We used immunohistochemical analyses and fractionation of the cellpopulations to demonstrate that the calcium regulated expression of Myf5 occurs in the mononucleated reserve cells, We conclude that Myf5 gene expression is regulated by a calcineurin- and NFAT-dependent pathway in the reserve cell population of myotube cultures, These results may provide importantinsights into the molecular mechanisms responsible for satellite cell activation and/or the renewal of the satellite cell pool following activation and proliferation.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 20/10/20 alle ore 05:54:07