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Titolo:
The p115-interactive proteins GM130 and giantin participate in endoplasmicreticulum-Golgi traffic
Autore:
Alvarez, C; Garcia-Mata, R; Hauri, HP; Sztul, E;
Indirizzi:
Univ Alabama, Dept Cell Biol, Birmingham, AL 35294 USA Univ Alabama Birmingham AL USA 35294 Cell Biol, Birmingham, AL 35294 USA Univ Basel, Biozentrum, Dept Pharmacol, CH-4056 Basel, Switzerland Univ Basel Basel Switzerland CH-4056 armacol, CH-4056 Basel, Switzerland
Titolo Testata:
JOURNAL OF BIOLOGICAL CHEMISTRY
fascicolo: 4, volume: 276, anno: 2001,
pagine: 2693 - 2700
SICI:
0021-9258(20010126)276:4<2693:TPPGAG>2.0.ZU;2-Q
Fonte:
ISI
Lingua:
ENG
Soggetto:
VESICULAR TRANSPORT; MEMBRANE-PROTEIN; INTERMEDIATE COMPARTMENT; REQUIRES USO1P; MATRIX PROTEIN; COPI VESICLES; HELA-CELLS; COMPLEX; FUSION; APPARATUS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
49
Recensione:
Indirizzi per estratti:
Indirizzo: Sztul, E Univ Alabama, Dept Cell Biol, McCallum Bldg,Rm 668,1530 3rd Ave S, Birmingham, AL 35294 USA Univ Alabama McCallum Bldg,Rm 668,1530 3rd Ave SBirmingham AL USA 35294
Citazione:
C. Alvarez et al., "The p115-interactive proteins GM130 and giantin participate in endoplasmicreticulum-Golgi traffic", J BIOL CHEM, 276(4), 2001, pp. 2693-2700

Abstract

The transport factor p115 is essential for endoplasmic reticulum (ER) to Golgi traffic. P115 interacts with two Golgi proteins, GM130 and giantin, suggesting that they might also participate in ER-Golgi traffic. Here, we show that peptides containing the GM130 or the giantin p115 binding domain andanti-GM130 and anti-giantin antibodies inhibit transport of vesicular stomatitis virus (VSV)-G protein to a mannosidase II-containing Golgi compartment, To determine whether p115, GM130, and giantin act together or sequentially during transport, we compared kinetics of traffic inhibition, Anti-p115, anti-GM130, and anti-giantin antibodies inhibited transport at temporallydistinct steps, with the p115-requiring step before the GM130-requiring stage, and both preceding the giantin-requiring stage. Examination of the distribution of the arrested VSV-G protein showed that anti-p115 antibodies inhibited transport at the level of vesicular-tubular clusters, whereas anti-GM130 and anti-giantin antibodies inhibited after the VSV-G protein moved to the Golgi complex. Our results provide the first evidence that GM130 and giantin are required for the delivery of a cargo protein to the mannosidaseII-containing Golgi compartment, These data are most consistent with a model where transport from the ER to the cis/medial-Golgi compartments requires the action of p115, GM130, and giantin in a sequential rather than coordinate mechanism.

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Documento generato il 23/01/21 alle ore 03:45:22