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Titolo:
Translesion DNA synthesis by yeast DNA polymerase eta on templates containing N-2-guanine adducts of 1,3-butadiene metabolites
Autore:
Minko, IG; Washington, MT; Prakash, L; Prakash, S; Lloyd, RS;
Indirizzi:
Univ Texas, Med Branch, Sealy Ctr Mol Sci, Galveston, TX 77555 USA Univ Texas Galveston TX USA 77555 ly Ctr Mol Sci, Galveston, TX 77555 USA
Titolo Testata:
JOURNAL OF BIOLOGICAL CHEMISTRY
fascicolo: 4, volume: 276, anno: 2001,
pagine: 2517 - 2522
SICI:
0021-9258(20010126)276:4<2517:TDSBYD>2.0.ZU;2-6
Fonte:
ISI
Lingua:
ENG
Soggetto:
XERODERMA-PIGMENTOSUM; SACCHAROMYCES-CEREVISIAE; BUTADIENE MONOXIDE; THYMINE DIMER; BYPASS; PURINE; DAMAGE; CELLS; GENE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
27
Recensione:
Indirizzi per estratti:
Indirizzo: Lloyd, RS Univ Texas, Med Branch, Sealy Ctr Mol Sci, 5-144 MRB,301 Univ Blvd, Galveston, TX 77555 USA Univ Texas 5-144 MRB,301 Univ Blvd Galveston TXUSA 77555 55 USA
Citazione:
I.G. Minko et al., "Translesion DNA synthesis by yeast DNA polymerase eta on templates containing N-2-guanine adducts of 1,3-butadiene metabolites", J BIOL CHEM, 276(4), 2001, pp. 2517-2522

Abstract

Yeast DNA polymerase eta can replicate through cis-syn cyclobutane pyrimidine dimers and 8-oxoguanine lesions with the same efficiency and accuracy as replication of an undamaged template. Previously, it has been shown that Escherichia coli DNA polymerases I, II, and III are incapable of bypassing DNA substrates containing N-2-guanine adducts of stereoisomeric 1,3-butadiene metabolites. Here we showed that yeast polymerase eta replicates DNA containing the monoadducts (S)-butadiene monoepoxide and (S,S)-butadiene diolepoxide N-2-guanines albeit at an similar to 200-300-fold lower efficiency relative to the control guanine, Interestingly, nucleotide incorporation opposite the (R)-butadiene monoepoxide and the (R,R)-butadiene diolepoxide N-2-guanines was similar to 10-fold less efficient than incorporation oppositetheir S stereoisomers, Polymerase eta preferentially incorporates the correct nucleotide opposite and downstream of all four adducts, except that it shows high misincorporation frequencies for elongation of C paired with (R)butadiene monoepoxide N-2-guanine. Additionally, polymerase eta does not bypass the (R,R)- and (S,S)butadiene diolepoxide N-2-guanine-N-2-guanine intrastrand cross-links, and replication is completely blocked just prior to the lesion. Collectively, these data suggest that polymerase eta can toleratethe geometric distortions in DNA conferred by the N-2-guanine butadiene monoadducts but not the intrastrand cross-links.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 06/04/20 alle ore 21:44:28