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Titolo:
Combinatorial control of cyclin B1 nuclear trafficking through phosphorylation at multiple sites
Autore:
Yang, J; Song, HB; Walsh, S; Bardes, ESG; Kornbluth, S;
Indirizzi:
Duke Univ, Med Ctr, Dept Pharmacol & Canc Biol, Durham, NC 27710 USA Duke Univ Durham NC USA 27710 Pharmacol & Canc Biol, Durham, NC 27710 USA
Titolo Testata:
JOURNAL OF BIOLOGICAL CHEMISTRY
fascicolo: 5, volume: 276, anno: 2001,
pagine: 3604 - 3609
SICI:
0021-9258(20010202)276:5<3604:CCOCBN>2.0.ZU;2-U
Fonte:
ISI
Lingua:
ENG
Soggetto:
XENOPUS OOCYTE; PROTEIN-KINASE; CDC25 PROTEIN; DNA-DAMAGE; LOCALIZATION; CELL; P34(CDC2); IMPORT; EXPORT; ACTIVATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
32
Recensione:
Indirizzi per estratti:
Indirizzo: Kornbluth, S Duke Univ, Med Ctr, Dept Pharmacol & Canc Biol, Box 3813,C370,C370 LSRC,Res Dr, Durham, NC 27710 USA Duke Univ Box 3813,C370,C370 LSRC,Res Dr Durham NC USA 27710
Citazione:
J. Yang et al., "Combinatorial control of cyclin B1 nuclear trafficking through phosphorylation at multiple sites", J BIOL CHEM, 276(5), 2001, pp. 3604-3609

Abstract

Entry into mitosis is regulated by the Cdc2 kinase complexed to B-type cyclins, We and others recently reported that cyclin B1/Cdc2 complexes, which appear to be constitutively cytoplasmic during interphase, actually shuttlecontinually into and out of the nucleus, with the rate of nuclear export exceeding the import rate (1-3). At the time of entry into mitosis, the import rate is increased, whereas the export rate is decreased, leading to rapid nuclear accumulation of Cdc2/cyclin Iii, Although it has recently been reported that phosphorylation of 4 serines within cyclin B1 promotes the rapid nuclear translocation of Cdc2/cyclin B1 at G(2)/M, the role that individual phosphorylation sites play in this process has not been examined (3, 4),We report here that phosphorylation of a single serine residue (Ser(113) of Xenopus cyclin B1) abrogates nuclear export of cyclin B1, This serine lies directly within the cyclin B1 nuclear export sequence and, when phosphorylated, prevents binding of the nuclear export factor, CRM1. In contrast, analysis of phosphorylation site mutants suggests that coordinate phosphorylation of all 4 serines (94, 96, 101, and 113) is required for the accelerated nuclear import of cyclin B1/Cdc2 characteristic of G(2)/M. Additionally, binding of cyclin B1 to importin-beta, the factor known to be responsible for the slow interphase nuclear entry of cyclin B1, appears to be unaffectedby the phosphorylation state of cyclin B. These data suggest that a distinct import factor must be recruited to enhance nuclear entry of Cdc2/cyclin B1 at the G(2)/M transition.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/09/20 alle ore 23:56:33