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Titolo:
Actin and actin-binding proteins in bovine spermatozoa: Potential role in membrane remodeling and intracellular signaling during epididymal maturation and the acrosome reaction
Autore:
Howes, EA; Hurst, SM; Jones, R;
Indirizzi:
Babraham Inst, Signalling Programme, Gamete Signalling Lab, Cambridge CB2 4AT, England Babraham Inst Cambridge England CB2 4AT Lab, Cambridge CB2 4AT, England
Titolo Testata:
JOURNAL OF ANDROLOGY
fascicolo: 1, volume: 22, anno: 2001,
pagine: 62 - 72
SICI:
0196-3635(200101/02)22:1<62:AAAPIB>2.0.ZU;2-A
Fonte:
ISI
Lingua:
ENG
Soggetto:
BOAR SPERMATOZOA; F-ACTIN; DEPOLYMERIZING FACTOR; INVITRO CAPACITATION; CAPPING PROTEIN; PLASMA-MEMBRANE; IN-VITRO; SPERM; LOCALIZATION; POLYMERIZATION;
Keywords:
thymosin beta 10; destrin; TS-ACP; actin-capping; sperm surface remodeling;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
42
Recensione:
Indirizzi per estratti:
Indirizzo: Howes, EA Babraham Inst, Signalling Programme, Gamete Signalling Lab, Cambridge CB2 4AT, England Babraham Inst Cambridge England CB2 4AT ridge CB2 4AT, England
Citazione:
E.A. Howes et al., "Actin and actin-binding proteins in bovine spermatozoa: Potential role in membrane remodeling and intracellular signaling during epididymal maturation and the acrosome reaction", J ANDROLOGY, 22(1), 2001, pp. 62-72

Abstract

The actin cytoskeleton influences a wide range of functions in nonmuscle somatic cells. including shape, movement. and interactions with extracellular matrices. The role of actin in mammalian male germ cells, however, particularly during post-testicular development, is not well understood. in this paper. we examine 1) the distribution of 3 actin-regulatory proteins (thymosin beta 10, destrin, and a testis-specific actin capping protein) involvedin controlling the balance between actin monomers (G-actin) and actin filaments (F-actin), and 2) the distribution and polymerization status of actinin bull spermatozoa during epididymal maturation and following acrosomal exocytosis. Results show that in fixed, permeabilized testicular spermatozoaall 3 regulatory proteins las determined by binding of specific antibodies) are localized primarily to the acrosomal domain but during epididymal maturation they become confined to the equatorial segment. Following ejaculation, however, they extend back into the acrosomal region. In spermatozoa induced to undergo an acrosome reaction with the calcium ionophore, A23187, further rearrangement occurs with destrin, thymosin beta 10, and TS-ACP appearing in the postacrosomal domain. Actin is also found over the acrosome of testicular spermatozoa with both G- and F-actin present, although the 2 forms show slightly different patterns of distribution. Subsequently, actin inthe sperm head is largely confined to the equatorial segment until F-actinappears in the postacrosomal domain of acrosome-reacted spermatozoa. This redistribution of actin and actin-regulatory proteins, coupled with changing levels of actin polymerization, suggest a continuing role for actin in both posttesticular sperm maturation and acrosomal exocytosis.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/11/20 alle ore 03:29:48