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Titolo:
Long-term expression of foreign genes in normal human epidermal keratinocytes after transfection with lipid/DNA complexes
Autore:
Zellmer, S; Gaunitz, F; Salvetter, J; Surovoy, A; Reissig, D; Gebhardt, R;
Indirizzi:
Univ Leipzig, Fac Med, Inst Biochem, D-04103 Leipzig, Germany Univ Leipzig Leipzig Germany D-04103 t Biochem, D-04103 Leipzig, Germany Univ Leipzig, Fac Med, Inst Anat, D-04103 Leipzig, Germany Univ Leipzig Leipzig Germany D-04103 Inst Anat, D-04103 Leipzig, Germany Russian Acad Sci, Inst Bioorgan Chem, Moscow 117871, Russia Russian Acad Sci Moscow Russia 117871 oorgan Chem, Moscow 117871, Russia
Titolo Testata:
HISTOCHEMISTRY AND CELL BIOLOGY
fascicolo: 1, volume: 115, anno: 2001,
pagine: 41 - 47
SICI:
0948-6143(200101)115:1<41:LEOFGI>2.0.ZU;2-N
Fonte:
ISI
Lingua:
ENG
Soggetto:
STEM-CELLS; IN-VITRO; NAKED DNA; SKIN; VIVO; INJECTION; DELIVERY; FATE;
Keywords:
cationic lipids; transplantation; air-liquid interface culture; serum-free;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
27
Recensione:
Indirizzi per estratti:
Indirizzo: Zellmer, S Univ Leipzig, Fac Med, Inst Biochem, Liebigstr 16, D-04103 Leipzig, Germany Univ Leipzig Liebigstr 16 Leipzig Germany D-04103 zig, Germany
Citazione:
S. Zellmer et al., "Long-term expression of foreign genes in normal human epidermal keratinocytes after transfection with lipid/DNA complexes", HISTOCHEM C, 115(1), 2001, pp. 41-47

Abstract

Normal human epidermal keratinocytes were isolated and cultivated in scrum-free medium. The expression of the integrin subunits alpha (6) and beta (1) indicated that a high number of keratinocytes from the stem cell system was present. These cells were transfected with complexes made of different cationic lipids and marker genes. Effectene showed a 20-fold higher transfection efficiency, compared to Lipofectin and Lipofectamine, and a similar low toxicity. The transfection protocol was optimised. A DNA/lipid ratio of 0.133 showed the highest transfection efficiency. Keratinocytes expressed the marker gene luciferase for 20 days. The maximum expression occurred after3-4 days, where individual patches of fluorescent keratinocytes were detected. Transfected keratinocytes, cultivated at the air-liquid interface, expressed the marker gene beta -galactosidase for at least 7 weeks.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/12/20 alle ore 01:42:21