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Titolo:
Epithelial sodium channel and the mineralocorticoid receptor in cultured rat Muller glial cells
Autore:
Golestaneh, N; De Kozak, Y; Klein, C; Mirshahi, M;
Indirizzi:
Fac Med Paris, INSERM E9912, F-75006 Paris, France Fac Med Paris Paris France F-75006 , INSERM E9912, F-75006 Paris, France Fac Med Paris, INSERM U450, Paris, France Fac Med Paris Paris FranceFac Med Paris, INSERM U450, Paris, France Fac Med Paris, IFR 58, Paris, France Fac Med Paris Paris FranceFac Med Paris, IFR 58, Paris, France
Titolo Testata:
GLIA
fascicolo: 2, volume: 33, anno: 2001,
pagine: 160 - 168
SICI:
0894-1491(200102)33:2<160:ESCATM>2.0.ZU;2-2
Fonte:
ISI
Lingua:
ENG
Soggetto:
RETINAL-PIGMENT EPITHELIUM; HORMONE-RECEPTOR; OCULAR-TISSUES; ION CHANNELS; GENERATION; TRANSPORT; EYE; NA;
Keywords:
eye; retina; Muller cells; epithelial sodium channel; aldosterone; mineralocorticoid receptor;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
36
Recensione:
Indirizzi per estratti:
Indirizzo: Mirshahi, M Fac Med Paris, INSERM E9912, 15 Rue Ecole Med, F-75006 Paris, France Fac Med Paris 15 Rue Ecole Med Paris France F-75006 s, France
Citazione:
N. Golestaneh et al., "Epithelial sodium channel and the mineralocorticoid receptor in cultured rat Muller glial cells", GLIA, 33(2), 2001, pp. 160-168

Abstract

Muller glial cells are the major non-neuronal cells of the retina. They are involved in retinal function and exert a profound influence on the function of retinal neurons. We present an in vitro study of the localization of the mineralocorticoid receptor (MCR) and of the epithelial sodium channel (ENaC) in rat Muller glial cells isolated from rat retina, using respectively, a polyclonal antiserum raised against the rat purified MCR, and a rabbitpolyclonal antibody against the 14-amino acid (aa) peptide QGLGKGDKREEQGL,which corresponds to the N-terminal region (44-58aa) of the alpha -subunitof the ENaC. In an immunocytochemical study using anti-MCR and anti-ENaC antibodies, the MCR was detected as a protein present in the cytoplasm and in the nucleus, whereas ENaC was detected as a membrane-bound protein. Reverse transcription-polymerase chain reaction (RT-PCR) analysis using specificprimers, 5'-CTGCCTTTATGGATGATGGT-3' (sense), 5'-GTTCAGCTCGAAGAAGA-3' (antisense) for ENaC and 5'-AGGCTACCACAGTCTCCCTG-3' (sense) and 5'-GCAGTGTAAAATCTCCAGTC-3' (antisense) for MCR, showed expression of the ENaC and MCR genesin Muller cells. The presence of ENaC and MCR was detected as the predicted bands of 520 bp and 843 bp, respectively. In both cases, 100% identity was observed between the sequences of rat Muller cell (RMC) PCR products and rat kidney. Interestingly, the basal levels of ENaC were increased in vitroby the MCR-specific hormone, aldosterone. Thus, our results strongly suggest that the Muller glial cells may play a role in the regulation of extracellular Na+ concentration, which could be regulated by steroid-mediated sodium uptake. (C) 2001 Wiley-Liss, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 13/12/18 alle ore 23:40:43