Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
TP53-dependent chromosome instability is associated with transient reductions in telomere length in immortal telomerase-positive cell lines
Autore:
Schwartz, JL; Jordan, R; Liber, H; Murnane, JP; Evans, HH;
Indirizzi:
Univ Washington, Dept Radiat Oncol, Seattle, WA 98195 USA Univ WashingtonSeattle WA USA 98195 Radiat Oncol, Seattle, WA 98195 USA Massachusetts Gen Hosp, Dept Radiat Oncol, Boston, MA 02114 USA Massachusetts Gen Hosp Boston MA USA 02114 at Oncol, Boston, MA 02114 USA Univ San Francisco, Dept Radiat Oncol, San Francisco, CA 94117 USA Univ San Francisco San Francisco CA USA 94117 San Francisco, CA 94117 USA Case Western Reserve Univ, Dept Radiat Oncol, Cleveland, OH 44106 USA CaseWestern Reserve Univ Cleveland OH USA 44106 Cleveland, OH 44106 USA
Titolo Testata:
GENES CHROMOSOMES & CANCER
fascicolo: 3, volume: 30, anno: 2001,
pagine: 236 - 244
SICI:
1045-2257(200103)30:3<236:TCIIAW>2.0.ZU;2-P
Fonte:
ISI
Lingua:
ENG
Soggetto:
WILD-TYPE P53; HUMAN FIBROBLASTS; G(1) ARREST; GENOMIC INSTABILITY; SPINDLE CHECKPOINT; GENE AMPLIFICATION; MUTANT P53; CYCLE; SENESCENCE; ABERRATIONS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
48
Recensione:
Indirizzi per estratti:
Indirizzo: Schwartz, JL Univ Washington, Dept Radiat Oncol, Boc 356069, Seattle, WA 98195 USA Univ Washington Boc 356069 Seattle WA USA 98195 WA 98195 USA
Citazione:
J.L. Schwartz et al., "TP53-dependent chromosome instability is associated with transient reductions in telomere length in immortal telomerase-positive cell lines", GENE CHROM, 30(3), 2001, pp. 236-244

Abstract

Telomere shortening in telomerase-negative somatic cells leads to the activation of the TP53 protein and the elimination of potentially unstable cells. We examined the effect of TP53 gene expression on both telomere metabolism and chromosome stability in immortal, telomerase-positive cell lines. Telomere length, telomerase activity, and chromosome instability were measured in multiple clones isolated from three related human B-lymphoblast cell lines that vary in TP53 expression; TK6 cells express wild-type TP53, WTKI cells overexpress a mutant form of TP53, and NH32 cells express no TP53 protein. Clonal variations in both telomere length and chromosome stability were observed, and shorter telomeres were associated with higher levels of chromosome instability. The shortest telomeres were found in WTKI- and NH32-derived cells, and these cells had 5- to 10-fold higher levels of chromosome instability. The primary marker of instability was the presence of dicentric chromosomes. Aneuploidy and other stable chromosome alterations were alsofound in clones showing high levels of dicentrics. Polyploidy was found only in WTKI-derived cells. Both telomere length and chromosome instability fluctuated in the different cell populations with time in culture, presumably as unstable cells and cells with short telomeres were eliminated from thegrowing population. Our results suggest that transient reductions in telomere lengths may be common in immortal cell lines and that these alterationsin telomere metabolism can have a profound effect on chromosome stability. (C) 2001 Wiley-Liss. Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 19/09/20 alle ore 18:45:33